石榴皮鞣质改善大鼠被动型Heymann肾炎作用研究  

Effect of Tannins from Pomegranate Rind on Passive Heymann Nephritis in Rats

作　　者：周本宏[1,2] 周静[1] 郭咸希[1] 汪静[1] 刘刚[1] 

机构地区：[1]武汉大学人民医院,武汉430060 [2]武汉大学药学院

出　　处：《中国药师》2015年第10期1629-1633,共5页China Pharmacist

基　　金：国家自然科学基金资助项目(编号:31170330)

摘　　要：目的:研究石榴皮鞣质对被动型Heymann肾炎大鼠的改善作用。方法:兔皮内多次免疫制备兔抗鼠Fx1A抗血清,然后尾静脉注射Fx1A抗血清,构建大鼠被动型Heymann肾炎(PHN)模型。将实验大鼠随机分为6组,为正常对照组、模型组、贝那普利组、石榴皮鞣质高、中以及低剂量组。造模成功后,每天用相应的药物进行灌胃给药治疗,持续5周,收集各组大鼠每周的尿液用于检测24 h尿蛋白。给药治疗结束后,收集各组大鼠的血清用于检测血清中的白蛋白、尿素氮、肌酐、总胆固醇和三酰甘油等指标;取肾脏,进行HE和Masson染色,观察石榴皮鞣质对PHN大鼠病理损伤的影响。结果:连续灌胃5周后,石榴皮鞣质可以明显降低被动型Heymann肾炎大鼠24 h尿蛋白含量(P<0.01),显著降低PHN大鼠血清肌酐(P<0.01)、尿素氮(P<0.01或P<0.05)、总胆固醇(P<0.01)以及三酰甘油(P<0.01或P<0.05)水平,并显著增高PHN模型大鼠血清白蛋白(P<0.01或P<0.05);HE和Masson染色结果表明,石榴皮鞣质对PHN模型大鼠病理损伤具有改善作用。结论:石榴皮鞣质对被动型Heymann肾炎大鼠具有改善作用。Objective：To investigate the therapeutic effect of tannins from pomegranate rind on passive Heymann nephritis （ PHN） in rats. Methods：Rabbits were with intradermal immunization for many times to obtain anti-Fx1A serum, and then PHN model in rats was established by tail intravenous injection of anti-Fx1A serum. The experimental rats were randomly divided into six groups, the nor-mal control group, model group, benazepril group, and tannins from pomegranate rind group respectively at high, medium and low dose. After the success of modeling, each rat was received intragastric administration for five weeks, and then the total of urine was collected once a week for the detection of 24-hour urine protein. At the end of treatment, serum was collected for the detection of serum albumin, blood urea nitrogen, creatinine, total cholesterol and triglyceride. The pathological changes of the renal tissue were observed using hematoxylin-eosin and Masson staining to observe the effect of tannins from pomegranate rind on PHN rats. Results：After the in-tragastric administration for five weeks, tannins from pomegranate rind could obviously reduce the level of 24-hour urine protein in PHN rats（P〈0. 01）, significantly reduce the levels of serum creatinine （P〈0. 01）, urea nitrogen （P〈0. 01,P〈0. 01,P〈0. 05）, total cholesterol （P〈0. 01） and triglyceride （P〈0. 01,P〈0. 01,P〈0. 05） in PHN rats, and increase the level of serum albumin （P〈0. 01,P〈0. 05,P〉0. 05）. HE and Masson staining results indicated that tannins from pomegranate rind could improve the pathologi-cal damage in PHN rats. Conclusion：Tannins from pomegranate rind has notable improvement for PHN in rats.

关 键 词：石榴皮 鞣质 HEYMANN肾炎 尿蛋白 

分 类 号：R285.5[医药卫生—中药学]