淫羊藿饮片指纹识别模式的建立与评价  

Establishment and Evaluation of Fingerprint Identification Pattern for Epimedium Pieces

作　　者：张振巍[1] 孙旭[2] 高媛[2] 刘忠良[2] 陈瑶[2] 张振兴[2] 石磊[1] 

机构地区：[1]解放军第155中心医院,河南开封475003 [2]济南军区联勤部药品仪器检验所

出　　处：《中国药师》2015年第10期1634-1637,共4页China Pharmacist

基　　金：济南军区后勤科研计划(编号:CJN10L067)

摘　　要：目的:建立淫羊藿饮片的综合质量评价模式,为其质量控制提供参考。方法:通过指纹识别模式的建立,对市售淫羊藿饮片指纹峰进行双向反馈,评价淫羊藿饮片整体质量。采用HPLC Agilent ZORBAX Eclipse XDB-C18(150 mm×4.6 mm,5μm)色谱柱,乙腈-水梯度洗脱,流速:1.0 ml·min-1,柱温:30℃。结果:初步建立了市售淫羊藿饮片HPLC指纹图谱,确定共有峰16个,市售淫羊藿饮片整体被分为两类,并且两类质量相差较大。结论:建立淫羊藿饮片指纹图谱的11批饮片相似度相差较大,不同产地、采集时间等因素对淫羊藿饮片整体质量影响较大。指纹评价模式能很好的反应出中药饮片质量的整体趋势,适合多成分中药及其制剂的综合质量评价。Objective：To establish a comprehensive quality evaluation pattern for epimedium pieces to provide reference for the quality control. Methods：The overall quality of Epimedium brevicornu Maxim was evaluated through the establishment of a fingerprint identification mode to perform the bifunctional feedback to the fingerprint peaks. The chromatographic separation was carried out on an Agilent ZORBAX Eclipse XDB-C18 （150 mm × 4. 6 mm,5 μm） column with the mobile phase of acetonitrile and water with gradient e-lution. The flow rate was 1. 0 ml＆#183;min1 , and the column temperature was maintained at 30℃. Results：The HPLC fingerprint of Epi-medium brevicornu Maxim decoction pieces containing 16 common peaks was obtained. The marketed Epimedium brevicornu Maxim de-coction pieces were divided into two categories and the quality between them showed significant differences. Conclusion：The similarity of 11 batches of epimedium herbs used to establish the fingerprints shows notable different, and the different habitats and sampling time exhibit significant influence on the overall quality. The fingerprint pattern can effectively reflect the overall trend of quality of Chinese herbal pieces, which is suitable for comprehensive quality evaluation of traditional Chinese medicines with multiple components and their relative preparations.

关 键 词：淫羊藿饮片 质量评价 高效液相色谱法 

分 类 号：TQ460.72[医药卫生—药物分析学]