HPLC法测定VEC-5脂质体药物含量及包封率  

Determination of Content and Entrapment Efficiency of VEC-5 Liposomes by HPLC

作　　者：孙冰雪[1] 陈国庆[1] 耿瑞[1] 骆海峰[1] 赵兴红[1] 陈妍[1] 

机构地区：[1]吉林大学生命科学学院,长春130000

出　　处：《中国药师》2015年第10期1642-1645,共4页China Pharmacist

基　　金：国家自然科学基金项目(编号:31270807)

摘　　要：目的：建立HIV-1病毒感染因子Vif抑制药VEC-5脂质体中VEC-5含量及包封率的HPLC检测方法。方法：采用冻干重建法制备VEC-5脂质体,超速离心法分离游离药物与脂质体,HPLC法测定脂质体中VEC-5的含量及包封率。结果：VEC-5在20-100μg·ml-1范围内线性关系良好（r=0.999 0）。平均回收率为100.25%,RSD为0.93%（n=9）。检测三批VEC-5脂质体样品的含量分别为98.63%、100.43%、102.65%,均在标示量的90%-110%之间;包封率分别为94.89%、93.68%、94.56%,均在90%以上,符合药典关于脂质体制剂包封率大于80%的要求。结论：该方法准确、可靠,可用于VEC-5脂质体中VEC-5含量及包封率的测定。Objective：To establish an HPLC method for the determination of content and entrapment efficiency of HIV-1 virus in-fection factor Vif inhibitor VEC-5 liposomes. Methods：VEC-5 liposomes were prepared by a method of freeze-drying and reconstruc-tion. The separation of free drug from the liposomes was achieved by ultracentrifugation, and an HPLC method was used to determine the content and entrapment efficiency of VEC-5 liposomes. Results：The linear range of VEC-5 was 20-100 μg·ml-1（r=0. 999 0）. The average recovery was 100. 25% and RSD was 0. 93%（n=9）. The content of three batches of VEC-5 liposomes was 98. 63%, 100. 43% and 102. 65%, respectively within the range of 90%-110%, and the entrapment efficiency was 94. 89%, 93. 68% and 94. 56%, respectively, which was above 90%. Conclusion：The method is accurate and reliable, which can be used to determine the content and entrapment efficiency of VEC-5 liposomes.

关 键 词：VEC-5 脂质体 高效液相色谱法 含量 包封率 

分 类 号：R944.9[医药卫生—药剂学]