针毛蕨治疗慢性非细菌性前列腺炎的活性部位研究  

Study on Active Fractions of Macrothelypteris Oligophlebia for Chronic Non-bacterial Prostatitis in Rats

作　　者：韩盼[1] 赖永继 阮金兰[2] 邓艾平[1] 王奕[1] 

机构地区：[1]武汉市中心医院药学部,武汉430014 [2]华中科技大学同济医学院药学院,武汉430030

出　　处：《中国药师》2015年第10期1645-1648,共4页China Pharmacist

基　　金：国家自然科学基金项目(编号:81173065)

摘　　要：目的:探讨针毛蕨甲醇提取物对慢性非细菌性前列腺炎(CNP)模型大鼠的治疗效果,初步确定其活性部位。方法:粉碎的针毛蕨根茎用甲醇提取,所得浸膏依次用氯仿和乙酸乙酯萃取后得到氯仿部位、乙酸乙酯部位和水部位;建立角叉菜胶诱导的CNP模型,将实验大鼠随机分成正常对照组、模型组、阳性对照组、甲醇提取物组、乙酸乙酯组、氯仿组和水部位组,测定各组的前列腺指数,利用ELISA试剂盒测定各组大鼠血清中白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、环氧合酶-2(COX-2)和前列腺素E2(PGE2)的含量,前列腺组织HE染色后进行病理学观察。结果:与模型组相比较,黄酮含量较高的乙酸乙酯组和甲醇提取物组大鼠的前列腺指数明显减小(P<0.01),前列腺组织病理学改善显著,细胞因子IL-10、TNF-α、COX-2和PGE2的含量显著降低(P<0.05或P<0.01),尤其是乙酸乙酯组。结论:针毛蕨对CNP具有较好的治疗效果,初步确定乙酸乙酯部位为其活性部位。Objective：To explore the effect of the methanol extract of Macrothelypteris oligophlebia on chronic non-bacterial prosta-titis （ CNP） in rats to confirm the active fractions. Methods：The powdered rhizomes of M. oligophlebia were soaked in methanol. The methanol extract was suspended in water and then extracted successively with chloroform and ethyl acetate to obtain chloroform fraction, ethyl acetate fraction and water fraction. Carrageenan-induced CNP in rats was established. The rats were randomly divided into the sham-operated control group, model group, positive control group, methanol extract group, ethyl acetate fraction group, chloroform fraction group and water fraction group. The anti-prostatitis effect was evaluated by the prostate index, and the pathological examination of prostate was performed using HE staining. The levels of interleukin-10 （IL-10）, tumor necrosis factor alpha （TNF-α）, cyclooxyge-nase-2 （COX-2） and prostaglandin E2（PGE2） were analyzed using ELISA kits. Results：The ethyl acetate fraction group and metha-nol extract group with high flavonoid content could significantly decrease prostate index （P〈0. 01） and the levels of IL-10, TNF-α, COX-2 and PGE2（P〈0. 05 or P〈0. 01）, and improve the prostate morphology when compared with the model group, especially with the ethyl acetate fraction group. Conclusion：The rhizomes of M. oligophlebia show promising therapeutic effect on CNP, and the ethyl acetate fraction is the active fraction.

关 键 词：慢性非细菌性前列腺炎 针毛蕨 甲醇提取物 乙酸乙酯部位 前列腺指数 细胞因子 

分 类 号：R285.5[医药卫生—中药学]