地榆鞣质提取物对TGF-β_1诱导人肾小管上皮细胞增殖的影响  

Influence of Sanguisorba Officinalis Tannins Extract on Proliferation of Human Renal Tubular Epithelia Cells Induced by TGF-β_1

作　　者：易慧兰 周本宏[1,2] 凃杰[2] 郭咸希[1] 吴玥[1] 

机构地区：[1]武汉大学人民医院药学部,武汉430060 [2]武汉大学药学院

出　　处：《中国药师》2015年第10期1668-1670,1673,共4页China Pharmacist

摘　　要：目的:通过观察地榆鞣质提取物(STE)对生长转化因子(TGF-β1)诱导人肾小管上皮细胞(HK-2)增殖的影响,探讨地榆在防治肾间质纤维化方面的作用。方法:将HK-2细胞用含10%胎牛血清的DMEM高糖培养基培养,实验分为5组:空白对照组、TGF-β1组(5 ng·ml-1TGF-β1)、干预1组(5 ng·ml-1TGF-β1+12.5μg·ml-1STE)、干预2组(5 ng·ml-1TGF-β1+25μg·ml-1STE)、干预3组(5 ng·ml-1TGF-β1+50μg·ml-1STE),孵育24 h。在倒置显微镜下观察各组细胞形态的改变,并通过CCK8法测定地榆鞣质对细胞增殖的影响。结果:TGF-β15 ng·ml-1能显著诱导人肾小管上皮细胞增殖,并促进其细胞形态向纤维化转变,相比于空白对照组具有显著差异(P<0.05);但与地榆鞣质提取物(STE)共同作用后,其促进细胞增殖的作用受到一定抑制(P<0.05),细胞形态也趋于正常,且呈剂量依赖性。结论:地榆鞣质提取物能抑制HK-2细胞的增殖,在一定程度上具有防止肾间质纤维化的作用。Objective：To study the preventive effect of Sanguisorba officinalis on renal fibrosis by observing the influence of San-guisorba officinalis tannins extract （STE） on the proliferation of human renal epithelia （HK-2） induced by transforming growth factor-β1 （ TGF-β1 ） . Methods：HK-2 cells were cultured in DMEM medium with high glucose containing 10% fetal bovine serum. The cul-tured cells were divided into 5 groups, including the blank control group, TGF-β1 group （5 ng· ml-1 TGF-β1）, intervention group 1 （5 ng·ml-1 TGF-β1 ＋12. 5μg·ml-1 STE）, intervention group 2 （5 ng·ml-1 TGF-β1 ＋25μg·ml-1 STE） and intervention group 3（5 ng·ml-1 TGF-β1 ＋50 μg·ml-1 STE）. The changes of cell morphology were observed under an inverted microscope and the in-fluence of SET on the cell proliferation was detected by CCK8 assay. Results： TGF-β1 could significantly induce the proliferation of HK-2 and promote the cell fibrosis with significant difference when compared with the control group （P〈0. 05）. However, after trea-ted with STE, the cell proliferation was inhibited obviously （P〈0. 05） and the cells morphology tended to be normal in a dose-depend-ent manner. Conclusion：STE can inhibit the proliferation of HK-2 and prevent renal fibrosis to some extent.

关 键 词：地榆鞣质提取物 生长转化因子 人肾小管上皮细胞 增殖 

分 类 号：R285.5[医药卫生—中药学]