络塞维改善D-半乳糖致亚急性衰老大鼠的学习记忆力的机制初探  

Preliminary Study on Protection Mechanism of Rosavin in Learning and Memory Ability in Subacute Aged Rats Induced by D-Galactose

作　　者：谭海龙[1] 师超[1] 卢婧[1] 

机构地区：[1]解放军海军总医院药剂科,北京100048

出　　处：《中国药师》2015年第10期1729-1732,共4页China Pharmacist

摘　　要：目的:观察络塞维改善D-半乳糖致亚急性衰老大鼠学习记忆力下降的作用,并初步研究其作用机制。方法:将48只SD大鼠随机分为对照组,模型组,阳性药物组,络塞维6、12、24 mg·g-1剂量组。除对照组外,所有大鼠颈部皮下注射D-半乳糖120 mg·kg-1·d-1造模。在给药进行4周后,采用Morris水迷宫测试各试验组大鼠学习记忆能力差异,血气分析仪测定大鼠脑中血氧分压(PO2),血氧浓度(O2)及血氧饱和度(Sa O2),并在试验结束后处死大鼠测定脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化酶(GSH-Px)活性和丙二醛(MDA)含量。结果:络塞维12、24 mg·g-1·d-1可显著缩短衰老大鼠在Morris水迷宫的潜伏期(P<0.01或P<0.05),并减少大鼠的游泳总距离和错误角度。同时,给予络塞维6,12,24 mg·g-1·d-1治疗衰老大鼠均能不同程度提高大鼠脑内PO2,O2和Sa O2浓度,SOD、CAT、GSH-Px活性及降低MDA含量,并具一定量效关系。结论:络塞维可改善D-半乳糖所致大鼠学习记忆下降现象,该作用可能与回升血氧含量,并保护SOD、CAT、GSH-Px等酶活性,降低MDA生成有关。Objective：To investigate the protection mechanism of rosavin in learning and memory ability in subacute aged rats in-duced by D-galactose. Methods：Totally 48 SD rats were randomly divided into the normal group, model group, positive drug group, and rosavin group respectively at the dose of 6, 12 and 24 mg·g-1 . Except the normal group, the other rats were with neck subcuta-neous injection of D-galactose 120 mg · kg-1 · d-1 . After 4-week drug administration, the learning and memory ability of rats was studied using Morris water maze. PO2 , SaO2 , the activity of superoxide dismutase （ SOD） , catalase（ CAT） and glutathione peroxidase （ GSH-Px） and the content of O2 and malonaldehyde（ MDA） of rats in vivo were determined at the end of the experiment. Results：The aged rats treated with rosavin（12 or 24 mg·kg-1·d-1） were with significant shortened latent period in Morris water maze（P〈0. 01 or P〈0. 05）, and with reduced total swimming distance and error angle. Meanwhile, rosavin（6,12 or 24 mg·kg-1·d-1） could im-prove the concentration of O2 ,PO2 and SaO2 , the activity of SOD, CAT and GSH-Px in brain, while decrease the content of MDA with certain dose-effect relationship. Conclusion：Rosavin can inhibit D-galactose induced learning and memory decrease in rats, and the effect may be related with the increase of oxygen content, enzyme activity protection of SOD, CAT, MAO and GSH-Px and decrease of MDA generation.

关 键 词：络塞维 学习能力 MORRIS水迷宫 抗衰老 

分 类 号：R285.5[医药卫生—中药学]