中药心得宁口服液对水负荷大鼠模型的影响  

Effect of Traditional Chinese Medicine Practolol Oral Liquid on Water Load Rat Model

作　　者：赵京生[1] 杨君[1] 易伟国[1] 刘弘[1] 张正臣[1] 

机构地区：[1]解放军第152中心医院,河南平顶山467000

出　　处：《中国药师》2015年第10期1810-1813,共4页China Pharmacist

摘　　要：目的:观察心得宁口服液对水负荷大鼠模型的影响。方法:取筛选合格的大鼠70只随机分为7组,每组10只;分别灌服心得宁口服液(20,10,5 ml·kg-1)、呋塞米片混悬液(0.033 5 mg·ml-1)、芪苈强心胶囊混悬液(0.03 g·ml-1)和等体积的蒸馏水(模型组)。空白组不灌服。将大鼠放入代谢笼,每隔1 h记一次尿量,共计5 h,收集给药后5 h的总尿量,测尿液中Na+、K+、Cl-的浓度。结果:与空白组比,模型组在2,3,5 h尿量均显著增加(P<0.01),总尿量也显著增加(P<0.01),尿液中K+浓度明显减少(P<0.05)、尿液中Na+、Cl-浓度有增加趋势,说明造水负荷模型成功。与模型组比,呋塞米可显著升高大鼠1,2,3,4,5 h尿量和总尿量(P<0.01),芪苈强心胶囊组尿液中K+浓度明显升高(P<0.05),呋塞米组尿液中K+浓度明显降低(P<0.05),大剂量心得宁口服液可显著升高大鼠5 h尿量(P<0.01)以及尿液中Na+、Cl-的浓度(P<0.01);中剂量心得宁口服液可明显升高大鼠3 h及5 h尿量和总尿量(P<0.05)及尿液中Cl-的浓度(P<0.05);小剂量心得宁口服液组中Na+、Cl-的浓度均显著降低(P<0.01)。与芪苈强心胶囊组比,心得宁口服液大、中、小剂量对1,2,3,4,5 h尿量和总尿量影响无明显差异(P>0.05);大剂量心得宁口服液可显著增加尿液中Na+浓度(P<0.01),中、小剂量心得宁口服液可明显增加尿液中Na+浓度(P<0.05);小剂量心得宁口服液可显著减少尿液中K+浓度(P<0.01);大、中剂量心得宁口服液可显著增加尿液中Cl-浓度(P<0.01),小剂量心得宁口服液可显著减少尿液中Cl-浓度(P<0.01)。与大剂量心得宁口服液组比,中、小剂量心得宁口服液对1h、2 h、3 h、4 h及5 h尿量影响无明显差异(P>0.05),但均可显著增加总尿量(P<0.01);中、小剂量心得宁口服液可显著较少尿液中Na+浓度(P<0.01)、可显著减少尿液中Cl-浓度(P<0.01)。结论:心得宁口服液有显著利尿作用且有量效关系。Objective： To investigate the influence of practolol oral liquid on the rat model of water load. Methods： Totally 70 qualified rats were selected and randomly divided into seven groups with 10 ones in each, and the rats were with intragastric administration respectively by practolol oral liquid （20,10 and 5 ml ·kg-1, respectively diluted 20 ml, 10 ml and 5 ml to 20 ml liquid with distilled water）, furosemide tablets suspension （0.033 5 mg · ml-1 ）, Qiliqiangxin capsule suspension（0.03 g · ml-1 ） and distilled water with the same voulme（model）. The rats were placed in metabolism cages. After the administration, the urine volume was recorded every 1 h for 5 hours, the total volume of urine was collected during the 5 h, and the concentration of Na＋ , K ＋ and Cl- in the urine was measured. Results： Compared with that in the blank group, the urine volume in 2 h, 3 h and 5 h after the administration in the model group was significantly increased（P 〈 0.01 ）, the total urine volume was also increased significantly（ P 〈 0.01 ）, the K＋ concentration was significantly reduced in the urine（ P 〈 0.05 ）, and there was an increasing trend of Na＋ and Cl - concentration in the ufine, which indicated that the water load model was made successfully. Compared with the model group, furosemide could significantly increase the urine volume in 1 h, 2 h, 3 h, 4 h and 5 h after the administration, and the total urine volume was increased as well （P 〈 0.01 ）. Qiliqiangxin capsule group could significantly increase the K ＋ concentration in the urine （P 〈 0.05 ） , and fumsemide could significantly reduce the K ＋ concentration in the urine （ P 〈 0.05 ）. High-dose praetolol oral liquid could significantly increase the urine volume in 5 h after the administration and the Na ＋ and Cl - concentration（ P 〈 0.01 ）, mid-dose praetolol oral liquid could significantly increase the urine volume in 3 h and 5 h after the administration and the total urine volume （ P 〈 0.

关 键 词：心得宁口服液 大鼠 水负荷模型 呋塞米片 芪苈强心胶囊 

分 类 号：R285.5[医药卫生—中药学]