构树黄酮诱导肝癌细胞凋亡的初步观察  

Preliminary Observation of the HepG2 cell Apoptosis Induced by Broussonetia papyrifera Flavonoids

作　　者：陈绍红[1] 陈训耿 何景淋 刘铀[1] 刘艳芬[2] 

机构地区：[1]广东海洋大学现代生化中心,广东湛江524088 [2]广东海洋大学农学院,广东湛江524088

出　　处：《安徽农业科学》2015年第29期32-34,共3页Journal of Anhui Agricultural Sciences

基　　金：广东省农业厅资助项目(0809035)

摘　　要：[目的]研究构树黄酮对肿瘤细胞的抑制效果。[方法]将处于对数生长期的Hep G2细胞接种于含有0、25、50、100、200、300μg/ml构树黄酮的DMEM培养基中,在37℃、5%CO2条件下分别培养24、48和72 h,采用MTT法测定构树黄酮对Hep G2细胞增殖的影响、用碘化丙啶(PI)染色法观察细胞形态的变化,并计算Hep G2细胞凋亡率。[结果]构树黄酮能显著抑制Hep G2细胞增殖,并呈现剂量和时间依赖性;构树黄酮能诱导Hep G2细胞凋亡,其最低有效浓度为25μg/ml;且Hep G2细胞凋亡率呈随着构树黄酮浓度增加及作用时间延长而升高。[结论]构树黄酮能抑制Hep G2细胞增殖并诱导其凋亡。[ Objective ] The research aimed to study the inhibitory effect of the total flavonoids of Broussonetia papyrifera on tumor cells. [ Method] HepG2 cells at logarithmic growth phase were seeded in the DMEM medium containing O, 25, 50, 100, 200, 300 μg/ml of the Broussonetia papyrifera flavonoids, and the cells were cultured at 37 ℃, 5 % CO2 conditions for 24, 48 and 72 h respectively. Then the inhibi- tory effect of the Broussonetia papyrifera flavonoids on HepG2 proliferation was detected by MTT method, the morphology changes of the ceils were observed by propidium iodide （PI） staining method, and the ratio of the apoptosised HepG2 ceils were calculated subsequently. [ Result ] The proliferation of HepG2 cells were inhibited by Broussonetia papyrifera flavonoids suplementation in the medium and showed dose and time dependent. Moreover, the apoptosis of HepG2 cells could be induced by Broussonetia papyrifera flavonoids, and the lowest effective concentra- tion of the flavonoids was 25 μg/ml, the apoptosis rate of HepG2 ceils were increased with Broussonetia papyrifera flavonoids concentration in- creased and treatment time prolonged. [ Conclusion ] The Broussonetia papyrifera flavonoids could inhibit proliferation and induce apoptosis of HeoG2 cells.

关 键 词：构树 黄酮化合物 HEPG2细胞 细胞凋亡 

分 类 号：S567[农业科学—中草药栽培]