热休克蛋白70mRNA表达及蛋白水平对职业性锰接触者的神经保护作用  

Expression of heat shock protein 70 and its mRNA in career exposure to manganese

作　　者：陈雯雯[1] 邵华[1] 迟名锋 张志虎[1] 单永乐[1] 邹微[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院职业卫生检测评价中心,济南250062 [2]山东省运动康复研究中心实验室

出　　处：《中华预防医学杂志》2015年第10期914-918,共5页Chinese Journal of Preventive Medicine

基　　金：国家“十一五”科技支撑项目（2006BAI06B02）

摘　　要：目的研究热休克蛋白70（HSPs70）mRNA表达及蛋白水平对职业性锰接触者的神经保护作用。方法于2008-2009年，采用横断面研究设计，应用分层随机抽样方法，在山东省某机车车辆厂选择接触锰浓度稳定、作业位置相对固定、工作1年以上的专职电焊作业人员作为暴露组，共180名；采用上述方法选择与暴露组民族、性别、年龄、出生地相似，最近3年内无锰职业接触的同机车车辆厂非电焊工人员作为对照组，共100名。以SYBR Green I嵌合Real-time PCR检测HSPs70mRNA含量表达；应用Western blot法检测HSPs70的蛋白含量；并应用火焰原子吸收法测定锰职业接触工人的空气锰个体接触剂量。用8h时间加权平均浓度（8h-TWA）表示锰个体接触浓度，根据空气锰工作场所职业接触限值（8h-TWA=0．15mg／m^3），将暴露组分为高暴露组（〉0．15mg／m^3）和低暴露组（〈0．15mg／m^3）结果暴露组个体锰接触剂量为（0．25±0．31）mg／m^3，高于对照组[（0．06±0．02）mg/m^3]（t=6．15，P=0．001）；高暴露组个体锰接触剂量为（0．42±0．34）mg／m^3，高于低暴露组[（0．09±0．07）mg／m^3]（t=9．80，P=0．001）。暴露组HSPs70mRNA及蛋白表达量分别为5．65±0．21、3．26±0．15，均高于对照组（0．41±0．03和1．32±0．12）（t值分别为18．91、8．68，P值均为0．001）；高暴露组的HSPs70mRNA及蛋白表达水平分别为6．48±0．37、3．67±0．26，亦均高于低暴露组（5．15±0．23和3．02±0．19）（t值分别为3．24、2．04，P值分别为0．003、0．043）。结论锰接触者外周血淋巴细胞HSPs70水平及HSPs70mRNA表达的增高与保护神经细胞免受锰刺激引起的脂质过氧化等损伤有关。Objective To analyze the expression levels of heat shock protein70 （HSPs70） and HSPs70 mRNA in different exposure to manganese, and research the neuroprotective effect on the career exposure to manganese. Methods From 2008 to 2009, with cross-sectional study design, and in a locomotive and rolling stock works, by stratified random sampling method, the exposed sample consisted of 180 welders from different welding shops and 100 unexposed in the last three years, non-welder controls with age-matched workers of similar socioeconomic status from the same industry. The control workers had not been exposed to neurotoxic chemicals. The mRNA expressions of four different metabolic enzyme were detected by SYBR Green I quantitative real-time polymerase chain reaction. The expression levels of the two enzymes mRNA in different exposure to manganese were analyzed. The expressions of HSPs70 were detected by Western blot. The concentration of air manganese was determined by GFAAS.The average concentration of 8 h time （Sh-TWA） was used to express the level of individual exposure to manganese, according to the air manganese workplace occupational exposure limit （8h-TWA=0.15 mg/m^3）, the exposed group is divided into high exposed group （〉0.15 mg/m^3） and low exposure group （〈0.15 mg/m^3）. Results The individuals exposed to manganese dose of exposed group （0.25±0.31） mg/m^3） was higher than the control group （（0.06 ± 0.02） mg/m^3） （t=6.15, P=0.001）; individuals exposed to manganese dose of high exposure group for （0.42±0.34） mg/m^3, which was higher than low exposure group （0.09±0.07） mg/m^3 （t=9.80, P=0.001）. HSPs70 mRNA and protein of exposure group （5.65 ± 0.21, 3.26± 0.15） were higher than the reference group （0.41 ±0.03, 1.32±0.12） （t=18.91, t=8.68, P=0.001）. HSP70 mRNA and protein of high exposure group（6.48±0.37, 3.67±0.26） were higher than the low exposure group （5.15±0.23, 3.02±0.19）（t= 3.24,t=2.01,P=0.003, P=0.043）. Conclusion The e

关 键 词：锰 蛋白质类 RNA 信使 蛋白印迹法 

分 类 号：R135.1[医药卫生—劳动卫生]