丙戊酸钠促进成纤维细胞重编程后表达心肌标志物的效率  

Sodium Valproate Promotes the Efficiency of Cardiomyocyte Markers Expression on Fibroblast Reprogramming

作　　者：陈有仁[1] 刘少飞[2] 邱汉婴[3] 庄霖鹏[1] 张长翼[1] 郑三晖[1] 周昱[4] 

机构地区：[1]汕头大学医学院第二附属医院心内科,广东汕头515041 [2]汕头大学医学院第二附属医院口腔科,广东汕头515041 [3]汕头大学医学院第一附属医院心内科,广东汕头515041 [4]汕头大学医学院第二附属医院神经内科,广东汕头515041

出　　处：《汕头大学医学院学报》2015年第3期152-154,162,F0004,共5页Journal of Shantou University Medical College

基　　金：广东省科技计划资助项目(2009B030801310)

摘　　要：目的:观察丙戊酸钠(VPA)对成纤维细胞表达心肌细胞标志物的促进作用,探讨VPA促进成纤维细胞重编程为心肌细胞的可能性。方法:构建慢病毒载体后,用慢病毒将Gata4、Mef-2c及Tbx5(GMT组)转染至小鼠胚胎成纤维细胞中,在此基础上添加VPA(GMT+VPA组),转染后培养21 d,用细胞免疫荧光、RT-PCR等方法检测该类细胞表达心肌细胞标志物蛋白和基因表达水平。结果:与未转染成纤维细胞(阴性对照组)比,GMT及GMT+VPA组都出现心肌特异性蛋白及基因表达,但远低于阳性对照组(P<0.05);GMT+VPA组心肌特异性蛋白及基因表达明显高于GMT组(P<0.05)。结论:VPA能提高成纤维细胞重编程后表达心肌细胞特异性蛋白及基因标志物,对于能否促进成纤维细胞转化为典型的心肌细胞,尚需进一步探讨。Objective：To observe the promotion effect of sodium valproate（VPA）on cardiomyocyte markers by fibroblasts,and to explore the possibility of promoting a reprogramming of fibroblasts into cardiomyocytes. Methods：The lentivirus vector was constructed. Three（Gata4,Mef-2c and Tbx5. GMT group）transcription factors transfected into the fibroblasts of mice. VPA（GMT＋VPA group）was added,culturing for 21 days after transfection. The protein and gene expression levels were detected by immunofluorescence staining and RT-PCR. Results：Compared with non-transfected fibroblasts（negative control group）,the cardiac-specific protein and gene expressions in the GMT and GMT＋VPA groups had emerged,but far less than the positive control group（P0.05）. The cardiac-specific protein and gene expressions in the GMT＋VPA group was significantly higher than those of GMT group（P0.05）. Conclusion：VPA is able to improve cardiac fibroblasts specific protein and gene markers after reprogramming,the ability to promote fibroblasts into cardiomyocytes typically needs to be explored.

关 键 词：重编程 成纤维细胞 心肌细胞 标志物 丙戊酸钠 

分 类 号：R34[医药卫生—基础医学]