高效液相色谱法测定益母草碱大鼠血浆浓度及其药代动力学研究  

Determination of Concentration of Leonurine in Rat Plasma by HPLC and Its Pharmacokinetics

作　　者：袁承军[1] 袁浩宇[2] 俞瑜[2] 王鹏[2] 

机构地区：[1]四川省绵阳市中心医院,四川绵阳621000 [2]核工业四一六医院药剂科,四川成都610051

出　　处：《中国药业》2015年第20期34-35,共2页China Pharmaceuticals

摘　　要：目的建立测定大鼠血浆中益母草碱质量浓度的高效液相色谱(HPLC)法,并研究益母草碱在大鼠体内的药代动力学特征。方法 8只SD大鼠尾静脉注射益母草碱后,采用HPLC法测定血浆中益母草碱的质量浓度,流动相为乙腈-0.02 mo L/L磷酸二氢钾(磷酸调p H=4.0)=60∶40,波长为277 nm,流速为1 m L/min,柱温为30℃。结果益母草碱在大鼠体内分布符合二室模型,主要药代动力学参数消除半衰期(t1/2α)为(0.46±0.13)h,t1/2β为(2.90±1.41)h,中心室分布容积(V)为(0.46±0.07)L/kg,药时曲线下面积(AUC0-t)为(70.65±12.39)mg/(h·L)。结论该方法简便、快速,可用于体内益母草碱的测定及药代动力学研究。Objective To establish the HPLC method to detect the mass concentration of leonurine in rat plasma,and the pharmacoki- neties was evaluated. Methods The mass concentration of leonurine in 8 rats was detected after intravenous injection of 20 mg/kg leonurine by HPLC. The mobile phase was acetonitrile-0. 02 moL/L mono potassium phosphate（adjusting to pH =4. 0 with phosphoric acid） = 60：40,at the flow rate of 1.0 mL/min and detected at 277 nm,the column temperature was 30 ℃. Results The process of leonurine in rats fit two-compartment model. The main pharmacokinetic parameters were followed： ti/2α was （0. 46 ±0.13）h, t1/2β was （2.90±1.41）h, V was（0. 46±0.07）L/kg, AUC（0-t） was （70.65±12.39） mg/（h · L）.Conelusion This method was accurate,stable and sensitive ,which can be used for the determination and pharmacokinetic of Leonurine.

关 键 词：益母草碱 高效液相色谱法 药代动力学 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]