定喘止咳糖浆中麻黄碱及伪麻黄碱的含量测定  

Determination of Ephedrine and Pseudoephedrine in Dingchuan Zhike Syrup

作　　者：付静[1] 朱月月 聂诗明[1] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065

出　　处：《中国药业》2015年第20期79-80,共2页China Pharmaceuticals

摘　　要：目的建立测定喘止咳糖浆含量的高效液相色谱（HPLC）法。方法色谱柱为UltimateXB—C18柱（250mm×4．6mm，5μm），流动相为0．02mol／L磷酸二氢钠缓冲液（pH=3．0，0．4％的三乙胺）-甲醇（96：4），流速0．9mL／min，枉温30℃，检测波长210nm。结果盐酸麻黄碱质量浓度在1．9344～30．9500μg／mL（r=1．0000）与峰面积线性关系良好，平均回收率为99．50％；盐酸伪麻黄碱质量浓度在2．4813—39．7000μg／mL（r=0．9999）与峰面积线性关系良好，平均回收率为96．57％（n=5）。结论该方法简便、准确，重复性好．适用于定喘止咳糖浆的质量控制。Objective To formulate the HPLC method of content determination of the Dingchuan Zhike Syrup. Methods The chromatog- raphy： Ultimate-XB C18 column（250 mm ×4.6 mm,5 μm）; Mobile phase： methanol-0. 02 mol/L sodium dihydrogen phosphate buffer （pH = 3. 0, containing 0. 4% of triethylamine） （96： 4）; flow rate： 0. 9 mL/min; column temperature： 30 ℃; detection wavelength： 210 nm. Results Ephedrine hydroehloride was linear in the range of 1.934 4-30.950 0 μg/mL（r=1.000 0）, the average recovery was 99. 50%. Pseudoephedrine hydrochloride was linear in the range of 2.481 3-39. 700 0 μg/mL（ r =0. 999 9）, the average recovery was 96. 57% （n =5）. Conclusion The method is simple and accurate, it also has good reproducibility. This method can be applied to con- trol the quality of the dingchuan zhike syrup.

关 键 词：定喘止咳糖浆 高效液相色谱法 麻黄碱 伪麻黄碱 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]