川芎硫磺熏蒸前后的傅里叶变换红外光谱鉴别  

Identification of Sun-dried and Sulfur-fumigated Chuanxiong Rhizoma by FTIR

作　　者：裴科[1] 蔡皓[1] 刘晓[1] 娄雅静[1,2] 宋晓庆[1] 乔凤仙[1] 蔡宝昌[1] 

机构地区：[1]南京中医药大学药学院/国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023 [2]江苏柯菲平医药股份有限公司药物研究所,江苏南京210014

出　　处：《中药材》2015年第7期1393-1399,共7页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81173546;30940093);江苏省自然科学基金(BK2009495);江苏省国际科技合作计划资助项目(BZ2011053);国家药典委员会资助项目(2015);江苏省普通高校研究生科研创新计划(KYLX_0975);江苏高校优势学科建设工程资助项目(2011ZYX2-001)

摘　　要：目的：评价硫磺熏蒸对川芎化学成分的影响。方法：运用傅里叶变换红外光谱法对川芎硫磺熏蒸前后的醇提物及水提物进行分析。红外光谱图采用Omnic 8.0软件采集;二阶导数图谱采用OPUS 6.5软件对红外光谱图进行平滑及求导处理;吸收波数采用SPSS软件进行主成分分析。结果：川芎经过硫磺熏蒸之后,在波数2 000-750 cm-1区间内变化明显,某些吸收峰削弱或消失,同时也有一些新的吸收峰出现。结论：硫磺熏蒸对川芎的化学成分有一定的影响,应用傅里叶变换红外光谱法并结合二阶导数和主成分分析可有效、直观、快速地鉴别硫熏和非硫熏川芎。Objective： To evaluate the sulfur-fumigation effects on the chemical constituents of Chuanxiong Rhizoma. Methods： Alcoholic and aqueous extracts of sun-dried and sulfur-fumigated Chuanxiong Rhizoma samples were analyzed by FTIR. FTIR spectra were acquired and disposed by software Omnic 8. 0,second derivative IR spectra were analyzed by software OPUS 6. 5 through smoothing and differentiation treatment to FTIR spectra,and the absorption frequencies of alcoholic and aqueous extracts of sun-dried and sulfur-fumigated Chuanxiong Rhizoma samples were assessed by using principal component analysis with software SPSS. Results： There were significant differences between sun-dried and sulfur-fumigated Chuanxiong Rhizoma samples in the region ranging from 2 000 cm- 1to 750cm- 1. Some absorption peaks weakened or disappeared,and some newly emerged after the processing of sulfur-fumigation. Conclusion：The processing of sulfur-fumigation can influence the effective constituents of Chuanxiong Rhizoma. The established method of FTIR combined with second derivative and principal component analysis has been proved to be an effective,intuitional and rapid approach to distinguish sun-dried and sulfur-fumigated Chuanxiong Rhizoma.

关 键 词：硫磺熏蒸 川芎 傅里叶变换红外光谱法 二阶导数 主成分分析 

分 类 号：R282.4[医药卫生—中药学]