瓜蒌薤白配伍变化对瓜蒌中槲皮素药动学的影响  

Influence of the Compatibility of Gualou and Xiebai on Pharmacokinetics of Quercetin in Gualou

作　　者：鄢海燕[1] 邹纯才[1] 魏美玲[1] 黄馨[1] 张艳红[1] 陈雪娇[1] 

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《中药材》2015年第7期1472-1475,共4页Journal of Chinese Medicinal Materials

基　　金：安徽省科技攻关项目(12010402089);安徽省大学生创新创业训练计划项目(AH201410368078);皖南医学院重点培育基金项目(WK2014Z04)

摘　　要：目的:采用HPLC-DAD测定瓜蒌薤白提取液及单味瓜蒌提取液灌胃后不同时间点小鼠血浆中槲皮素的浓度,探讨瓜蒌薤白配伍对瓜蒌中槲皮素药动学的影响。方法:血浆用乙腈除蛋白后,以乙酸乙酯萃取3次,氮气挥干乙酸乙酯,残渣用甲醇溶解,HPLC分析测定。采用DAS 2.0数据处理软件及SPSS 11.5统计软件分别进行药动学参数计算及比较。结果:瓜蒌薤白提取液及单味瓜蒌提取液给小鼠灌胃后,槲皮素在小鼠血浆中的药代动力学过程符合二室模型,但两组t1/2α、t1/2β、AUC0-t、AUC0-∞、Cmax、tmax、Ka、K10、K12、K21、CL/F、V1/F药动学参数差异有统计学意义(P<0.05或P<0.01)。结论:瓜蒌薤白汤中薤白对瓜蒌中槲皮素的药动学特征具有显著影响,可促进槲皮素的吸收分布,减缓其在体内的消除,提高其生物利用度。Objective： To determine the concentration of quercetin in mouse plasma by HPLC-DAD in different time after oral administration with Gualou Xiebai decoction and Gualou decoction,and to explore the influence of the compatibility of Gualou and Xiebai on pharmacokinetics of quercetin in Gualou. Methods： The plasma sample was simply deproteinized with acetonitrile,extracted three times by aceticether,evaporated to dryness with a gentle N2 stream,dissolved with methanol and determined by HPLC. The pharmacokinetic parameters were separately calculated and compared with data processing software DAS 2. 0 and SPSS 11. 5 software. Results： After oral administration with Gualou Xiebai decoction and Gualou decoction,the pharmacokinetics of quercetin in mouse plasma accorded with the two-compartment model,and t1 /2α,t1 /2β,AUC0-t,AUC0-∞,Cmax,tmax,Ka,K10,K12,K21,CL/F and V1/ F all had significant differences（ P〈 0. 05 or P〈 0. 01）. Conclusion： Xiebai in Gualou Xiebai decoction has a remarkable effect on pharmacokinetic character of quercetin in Gualou,it can promote the absorption and distribution and reduce the elimination of quercetin,in vivo,as well as increase the bioavailability of quercetin.

关 键 词：瓜蒌薤白 配伍 槲皮素 药动学 

分 类 号：R285.5[医药卫生—中药学]