实时荧光定量PCR技术及其在几类病原体检测中的应用  

Real-time fluorescent quantitative PCR and its application in pathogen detection

作　　者：李海红[1] 侯锡苗[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西省杨凌712100

出　　处：《职业与健康》2015年第18期2586-2589,共4页Occupation and Health

基　　金：国家自然科学研究基金资助项目(项目编号:11304252)

摘　　要：实时荧光定量PCR是传统PCR技术与光谱技术相结合而发展起来的核酸定量技术,它具有特异性强、重复性好和PCR污染少等优点。作者对实时荧光定量PCR技术的原理、影响因素以及在临床病原体的快速检测和诊断应用方面进行了综述。Real-time fluorescent quantitative PCR （FQ-PCR） is technique of nucleic acid quantification that is developed on the basis of conventional PCR technique combined with spectroscopic techniques. It has characteristic of strong specificity, excellent repeatability and less PCR contamination. The authors of the article review the theory of FQ-PCR, affecting factors, and applications in rapid pathogen detection and diagnosis.

关 键 词：实时荧光定量PCR 水解探针 影响因素 病原体 应用 

分 类 号：R446[医药卫生—诊断学]