HPLC测定续断中马钱苷酸、马钱苷和当药苷的量  

Determination of loganic acid, chiratin, and loganin in Dipsaci Radix

作　　者：张芬[1,2] 黄文华[2] 孙欣光[2] 郭宝林[2] 陈安家[1] 

机构地区：[1]山西医科大学,山西太原030001 [2]中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京100193

出　　处：《中草药》2015年第17期2632-2634,共3页Chinese Traditional and Herbal Drugs

基　　金：国家科技重大专项资助项目(2011ZX09201-201-05);中国医学科学院药用植物研究所创新团队发展计划资助项目

摘　　要：目的建立HPLC法测定续断中马钱苷酸、马钱苷和当药苷的分析方法,通过对多个产地续断的分析,完善续断药材的质量评价方法。方法 Venusil MP C18色谱柱（250 mm×4.6 mm,5μm）,0.1%H3PO4（A）-乙腈（B）梯度洗脱：0~10 min,8%~9%B;10~30 min,9%~11%B;30~36 min,11%~100%B;体积流量1 m L/min,柱温25℃。结果马钱苷酸、马钱苷和当药苷得到很好分离,并分别在18.4~368.2、2.02~40.4、17.5~349.6μg/m L内线性关系良好;提取方法的回收率分别为99.34%、99.19%、101.61%。结论马钱苷酸、当药苷和马钱苷为续断中的主要环烯醚萜苷类成分,同时测定续断中3种环烯醚萜苷类成分的方法简便、快捷。各主产地续断中马钱苷酸、当药苷和马钱苷的范围分别为20.4~186.8、26.4~177.7和1.9~13.2 mg/g,3个成分之和在不同样品之间变化不大,建议作为续断药材质量控制标准。Objective To establish a method for the determination of loganic acid, chiratin, and loganin in Dipsaci Radix and improve the quality evaluation by analyzing Dipsaci Radix from different habitats. Methods The analysis was performed on Venusil MP C18column（250 mm × 4.6 mm, 5 μm） with mobile phase consisting of 0.1% H3PO4-acetonitrile for gradient elution： 0—10 min, 8%—9% B; 10—30 min, 9%—11% B; 30—35 min, 11%—100% B. The flow rate was 1 m L/min and the column temperature was 25 ℃. Results The results showed that loganic acid, chiratin, and loganin were well separated with the good linearity in 18.4—368.2, 2.02—40.4, and 17.5—349.6 μg/m L, respectively. The average recoveries of the three iridoid glycosides were 99.34%, 99.19%, and 101.61%. Conclusion Loganic acid, chiratin, and loganin are the main iridoid glycosides in Dipsaci Radix. The method can easily be applied to the content determination of loganic acid, chiratin, and loganin in Dipsaci Radix quickly. The content ranges of loganic acid, chiratin, and loganin are 20.4—186.8, 26.4—177.7, and 1.9—13.2 mg/g, respectively, and the sum of the three iridoid glycosides shows no significant differences in various Dipsaci Radix from different habitats, and proposes to evaluate the quality of Dipsaci Radix.

关 键 词：续断 HPLC 环烯醚萜苷 马钱苷酸 当药苷 马钱苷 

分 类 号：R286.6[医药卫生—中药学]