裴氏升血颗粒含药血清对K562/ADR细胞p170表达的影响  

The effect of PG to the expression of p170 on the K562/ADR cells

作　　者：吴玉强[1] 薛文翰[2] 

机构地区：[1]甘肃中医药大学 [2]甘肃省肿瘤医院中西结合科,甘肃兰州730050

出　　处：《甘肃医药》2015年第10期730-732,共3页Gansu Medical Journal

基　　金：甘肃省中医药管理局科研项目(编号:GZK-2008-13)

摘　　要：目的:观察裴氏升血颗粒(PG)含药血清对人白血病细胞株K562/ADR细胞p170蛋白表达水平的影响。方法:采用MTT法测定不同浓度含药血清对K562细胞系的细胞毒作用,用流式细胞仪检测非细胞毒性浓度含药血清处理后K562/ADR细胞膜表面p170表达变化。结果:不同浓度含药血清对K562/ADR细胞无明显细胞毒作用,非细胞毒性含药血清能明显下调K562/ADR细胞p170蛋白的表达。结论:裴氏升血颗粒能够提高化疗疗效,其机制可能与下调p170蛋白表达有关。Objective：To investigate the effect of PG herbal serum to the expression level of p170 protein on the human leukemia K562/ADR cells. Methods： Testing the cytotoxic effect of different herbal serum to K562/ADR cells line by MTT method, using Flow cytometry to determine the expressive and functional changes of p170 on K562/ADR cells membrane dealed with the herbal serum at non-cytotoxic concentration. Results：Different herbal serum had no obvious cytotoxic effect to K562/A02 cells, non-cytotoxic concentration of herbal serum can significantly lowered the expression of p170 protein on the K562/ADR cells. Conclusion： PG could improve the curative effect of chemotherapy, and its mechanism may be related to descend the expression of p170.

关 键 词：裴氏升血颗粒 K562/ADR细胞株 P170蛋白 

分 类 号：R285.5[医药卫生—中药学]