益肾养阴合剂对MRL/lpr小鼠狼疮性肾炎的保护作用及部分机制研究  

Study on the Protective Effect and Mechanism of Yishen Yangyin Mixture on Lupus Nephritis of MRL/lpr Mice

作　　者：吴洋[1] 张巍琼[2] 李东云[2] 粟荣[1] 

机构地区：[1]云南省中医医院,云南昆明650021 [2]云南中医学院,云南昆明650500

出　　处：《云南中医中药杂志》2015年第9期46-48,共3页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：国家中医药管理局十一五中医痹病学重点学科

摘　　要：目的探讨益肾养阴合剂对肾功、肾脏病理变化及对P-STAT3表达的影响,揭示其发挥疗效的作用机制,为益肾养阴合剂治疗狼疮性肾炎(LN)提供理论依据。方法两种种系相同不同品种的50只小鼠随机分5组:正常组C57BL/6J小鼠(0.9%生理盐水);MRL/1pr小鼠根据尿蛋白含量随机分为4组:模型组、中药组、中药联合激素组、激素组,连续给药4周后观察P-STAT3、尿蛋白含量、血肌酐、尿素氮及肾脏病理变化。结果在给药第一周,与模型组相比,尿蛋白含量在各治疗组之间无明显差异(P>0.05)。在第2、3、4周时,与模型组相比,各治疗组的尿蛋白、血肌酐、尿素氮含量均有显著下降(P<0.05),中药联合激素组的尿蛋白含量最低(P<0.05)。给药处理后,p-stat3的表达均呈下降趋势,中药组、激素组的p-stat3表达相比模型组有所减少(P<0.05);中药联合激素组的降低情况最为显著(P<0.05)。HE染色三个治疗组肾组织局部轻度系膜细胞增生,肾小球炎症细胞浸润减少。结论 1.益肾养阴合剂能改善MRL/lpr小鼠的肾功能及肾脏病理,且联合激素肾功能改善更明显。2.下调磷酸化的STAT3的表达可能是益肾养阴合剂治疗狼疮性肾炎的作用靶点之一。Objective： To study the effect ofYishen Yangyin Mixture on renal function, renal pathological changes and P -STAT3 presentation and reveal its clinical mechanism in order to provide a theoretical basis for the treatment of lupus nephritis （LN）. Methods ： Two germ lines of different kinds of 50 mice were randomly divided into five groups. Normal C57BL/6J mice （0.9% saline） and MRL/1 pr mice were randomly divided into four groups according to urinary protein content, a model group, a TCM group, a TCM combined hormone group and a hormone group and were administrated for 4 weeks to observe their P - STA33, urine protein, serum creatinine, blood urea ni- trogen and renal pathological changes. Results： In the 1st week administration, compared with the model group, the urine protein of each group was no significant difference （P 〉 0.05）. In the 2nd, 3rd and 4th week administration, compared with the model group, the urine protein, serum creatinine and urea nitrogen of each group significantly decreased （P 〈 0.05）, among which the urine protein of the TCM combined hormone group was lowest （P 〈 0.05 ）. After the administration, p - stat3 presentation showed a downward trend. The p - star3 presentation of the TCM group and the hormone group decreased when compared with that of the model group （ P 〈 0.05 ）. The p - stat3 of the TCM combined hormone group decreased most significantly （P 〈 0.05 ）. HE staining of renal tissue of three treatment groups showed local mild mesangial cell proliferation and glomerular inflammatory cell infiltration reduction. Conclusions： A. Yishen Yangyin Mixture can improve the renal function and renal pathology of MRL/lpr mice, and improve more obviously when combined hormone. B. The reduced phosphorylation STAT3 may be one of the targets for the treatment of lupus nephritis.

关 键 词：益肾养阴合剂 狼疮性肾炎 MRL/LPR小鼠 P-STAT3 病理 

分 类 号：R285.5[医药卫生—中药学]