流动注射化学发光法测定头孢磺啶钠  

Flow Injection Chemiluminescence Determination of Cefsulodine Sodium

作　　者：翟倩[1] 何云[1] 李晓璐[1] 葛闯[1] 易钢[1] 

机构地区：[1]重庆医科大学检验医学院临床检验诊断学教育部重点实验室,重庆400016

出　　处：《分析科学学报》2015年第5期731-733,共3页Journal of Analytical Science

摘　　要：在碱性介质中,头孢磺啶钠对K3[Fe（CN）6]与鲁米诺化学发光体系具有明显的增强作用,据此建立了流动注射化学发光测定头孢磺啶钠的新方法。在选定的最佳实验条件下,化学发光增加强度与头孢磺啶钠的浓度在5.0×10-7~2.0×10-5 g/mL范围内呈良好的线性关系,检测限为3.2×10-7 g/mL。对4.0×10-6 g/mL头孢磺啶钠平行测定11次,相对标准偏差为2.25%。该方法可用于头孢磺啶钠粉末状药剂的测定。In this study,cefsulodine sodium was found to enhance the chemiluminescence of the luminolpotassium ferricyanide reaction system in alkaline condition.According to this fact,a new flow injection chemiluminescence method for the determination of cefsulodine sodium was developed.Under the optimum conditions,the linear range for cefsulodine sodium was 5.0×10-7-2.0×10-5 g/mL,and the limit of detection was 3.2×10-7 g/mL.The relative standard deviation（n=11）was 2.25% for the determination of 4.0×10-6 g/mL cefsulodine sodium.The method has been applied to the determination of cefsulodine sodium powdery reagent and the results were satisfactory.

关 键 词：化学发光 流动注射 头孢磺啶钠 鲁米诺 铁氰化钾 

分 类 号：O657.39[理学—分析化学]