丹芪偏瘫胶囊中羚羊角用人工牛黄替代的初步研究  

Preliminary study on substitution of Antelope Horn in Danqi Piantan capsule with artificial bezoar

作　　者：王进博[1,2] 陈涛[1] 李正[1] 张艳军[2] 崔维利[1] 李进[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津中医药大学,天津300193

出　　处：《中国中药杂志》2015年第18期3616-3622,共7页China Journal of Chinese Materia Medica

基　　金：天津市科技创新专项(06FZZDSH00408);天津市西青区科技创新专项计划项目(XQCXZX2012-006);天津市"杀手锏"产品及研发项目(XQKC2013-022)

摘　　要：为研究丹芪偏瘫胶囊(DPC)及其羚羊角替代方(DPCAS)对脑缺血的保护作用,初步探讨人工牛黄替代羚羊角的可能性,该文采用左侧大脑中动脉栓塞(MCAO)模型,将150只SD大鼠随机分为假手术组,模型组,DPC(0.246 g·kg-1·d-1)组,丹芪偏瘫胶囊去羚羊角(DPCRA,0.246 g·kg-1·d-1)组,丹芪偏瘫胶囊去羚羊角人工牛黄翻倍(DPCBD,0.246 g·kg-1·d-1)组。于第5天给药后1 h制备MCAO模型。术后24 h,眼内眦取血,测定血清超氧化物歧化酶(SOD)活性、内皮素(ET)含量。术后72 h取脑组织,氯化三苯基四氮唑(TTC)染色法测定大脑梗死面积、测定脑指数。荧光定量PCR方法检测脑组织bcl-2,caspase-3,IL-1β,P-选择素,E-选择素,ICAM-1 mRNA的表达,免疫组化方法检测缺血半暗区ICAM-1,IL-1β,TNF-α,IL-6的表达。结果,与模型组比较,DPCBD和DPC组实验结果基本一致,均能明显改善脑梗死指数和脑指数(P<0.05),均可明显增强大鼠血清SOD活性(P<0.05);均可在一定程度上降低大鼠血清ET水平;均可降低脑组织caspase-3的表达(P<0.05),升高bcl-2的表达(P<0.05);均可降低脑组织IL-1β,P-选择素,E-选择素,ICAM-1 mRNA的表达(P<0.05),均能减少缺血半暗区ICAM-1,IL-1β,TNF-α,IL-6阳性细胞的表达(P<0.05)。DPCRA组对各指标的影响明显不如DPCBD和DPC组。表明DPCBD与DPC疗效相当,提示丹芪偏瘫胶囊中羚羊角可用人工牛黄进行替代。To study the protective effect of Danqi Piantan capsule（ DPC） and its antelope horn substitution（ DPCAS） on the cerebral ischemia,in order to preliminary study the possibility of replacing antelope horn with artificial bezoar. In this study,the left middle cerebral artery occlusion（ MCAO） was adopted. Totally 150 SD rats were randomly divided into 5 groups： the sham operation group,the model group,the Danqi Piantan capsule（ DPC） group（ 0. 246 g·kg^- 1·d^- 1）,the Danqi Piantan capsule without antelope horn（ DPCRA） group（ 0. 246 g·kg^- 1·d^- 1the Danqi Piantan capsule without antelope horn and with double artificial bezoar（ DPCDB） group（ 0. 246 g·kg^- 1·d^- 1 The MCAO model was prepared 1 h later after the administration on the 5thday. At 24 h after the operation,the inner canthus blood was collected to determine the serum superoxide dismutase（ SOD） activity and the endothelin（ ET） content. At 72 h after the operation,the cerebral infarct size and the cerebral index were determined by TTC-staining. The fluorescent quantitative PCR method was used to detect brain Bcl-2,Caspase-3,IL-1β,P-selectin,E-selectin,ICAM-1 mRNA expressions. The mmunohistochemical method was used to detect ICAM-1,IL-1β,TNF-α,IL-6 expressions in ischemic penumbra. According to the results,compared with the model group,DPCDB and DPC groups showed almost consistent results,indicating both of the two group can significantly improved cerebral infarction index and cerebral index（ P〈0. 05）,increase the serum SOD activity（ P〈0. 05）,decrease the serum ET level and Caspase-3 expression,IL-1β,P-selectin,E-selectin,ICAM-1 mRNA expressions in brain tissues（ P〈0. 05） and expressions of ICAM-1,IL-1β,TNF-α,IL-6 positive cells in ischemic penumbra（ P〈0. 05） and increase the Bcl-2 expression（ P〈0. 05）. The DPCRA group showed much lower impacts on indexes than DPCDB and DPC groups. This suggests that DPCDB and DPC reveal similar efficacies and antelope horn in Danqi Piantan capsu

关 键 词：丹芪偏瘫胶囊 丹芪偏瘫胶囊去羚羊角 丹芪偏瘫胶囊去羚羊角人工牛黄翻倍 MCAO 脑梗死 抗氧化 细胞凋亡 炎症反应 

分 类 号：R285.5[医药卫生—中药学]