醋甘遂泻水逐饮功效活性部位筛选  

Filtration of active fractions with function of expelling water retention with drastic purgative from Kansui Radix stir-baked with vinegar

作　　者：曹亮亮[1] 王文晓[1] 张桥[1] 张丽[1] 丁安伟[1] 窦志华[1] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023

出　　处：《中国中药杂志》2015年第18期3655-3659,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973940;81373972);江苏省"六大人才高峰"项目(2010-YY-009);江苏高校优势学科建设工程项目(ysxk-2014)

摘　　要：考察醋甘遂不同极性部位对癌性腹水模型大鼠泻水逐饮的功效。以癌性腹水模型大鼠为研究对象,呋塞米为阳性药,分组连续7 d灌胃醋甘遂各极性部位,考察其对癌性腹水模型大鼠尿量、腹水量、尿钠钾氯离子水平、尿液p H、肾素-血管紧张素Ⅱ-醛固酮系统(RAAS)的影响。结果显示,与模型组比较,醋甘遂乙酸乙酯部位可显著增加癌性腹水模型大鼠尿量(P<0.05),减少腹水生成,降低尿中钠钾氯离子水平(P<0.05,P<0.01)、尿液p H(P<0.05)及血清肾素、血管紧张素Ⅱ、醛固酮含量(P<0.01)。其中石油醚部位和正丁醇部位作用程度不及乙酸乙酯部位,水部位对模型大鼠各环节的作用较弱。结果表明,醋甘遂乙酸乙酯部位对癌性腹水模型大鼠有显著的利水作用,可缓解水液电解质紊乱和体液酸碱失衡,调节肾素-血管紧张素Ⅱ-醛固酮系统,为醋甘遂泻水逐饮的功效部位。To study the function of expelling water retention with drastic purgative of different polarities of Kansui Radix stir-baked with vinegar on the cancerous ascites model rats,the furosemide was taken as positive control drug,and the cancerous ascites model rats were respectively orally administered with different polarities of Kansui Radix stir-baked with vinegar for 7 d. The amount of urine and ascites,the level of urinary sodium,potassium,chloride ion and p H,and the content of PRL1,AⅡ,ALD in serum were investigated. Compared with model groups,ethyl acetate extract group showed a decreasing trend in ascites; the amount of urine of showed a significant increase（ P〈0. 05）; the level of urinary sodium,potassium,chloride ion（ P〈0. 05,P〈0. 01）,p H（ P〈0. 05）,and the content of PRL1,AⅡ,ALD in serum all showed a significant decrease（ P〈0. 01）. The effects of petroleum ether extract and n-butanol extract were weaker than that of ethyl acetate extract. The water exact was the weakest. The results showed that ethyl acetate extract is the active part of Kansui Radix stir-baked with vinegar on the function of expelling water retention with drastic purgative on the cancerous ascites model rats,alleviating the water-electrolyte disorder and body fluid acid-base imbalance,regulating the renin angiotensin aldosterone system.

关 键 词：醋甘遂 癌性腹水 泻水逐饮 功效部位 筛选 

分 类 号：R285.5[医药卫生—中药学]