猪流行性腹泻病和猪圆环病毒病混合感染的诊治  

Diagnosis and Treatment of Porcine Epidemic Diarrhea Co-infecting with Porcine Circovirus Disease

作　　者：刘洋[1,2] 库旭钢[1,2] 刘晓丽[2] 何启盖[1,2] 

机构地区：[1]农业微生物学国家重点实验室,武汉430070 [2]华中农业大学动物医学院,武汉430070

出　　处：《中国畜牧兽医》2015年第10期2753-2761,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家生猪产业技术体系(CARS-36)

摘　　要：2014年2月,突遇降雪天气,某规模化猪场2 000头母猪中约100头母猪所产新生仔猪集中在1周龄内出现大批死亡现象,死亡前均出现呕吐、消瘦及水样稀粪症状。通过采用现场调查、实验室病理解剖、病理组织学观察、免疫组化、RT-PCR、测序、ELISA检测等方法,本试验确定该病是猪流行性腹泻病毒(PEDV)变异野毒株感染引起,同时混合感染猪圆环病毒2型(PCV2)。通过采取母猪群紧急接种疫苗、仔猪口服卵黄抗体及加强保温、消毒等综合措施,该病迅速得到控制。In February 2014,piglets of a certain 100 sows,fed in a large-scale pig farm which had2 000 sows,died acutely and densely in one week with vomit,emaciation and lethal watery diarrhea after sudden snowy weather.By field survey,pathological anatomy,histopathological observation,immunohistochemistry,reverse transcription-polymerase chain reaction（RT-PCR）,sequencing and enzyme-linked immune sorbent assay（ELISA）,porcine epidemic diarrhea virus（PEDV）,co-infecting with porcine circovirus 2（PCV2）,was confirmed to be the major agent.By adopting measures,including emergency vaccination of sows,egg yolk antibody oral inoculation of piglets,and insulation and disinfection,the disease was successfully and speedily controlled.

关 键 词：猪流行性腹泻病毒 RT-PCR S基因 诊断 

分 类 号：S852.6[农业科学—基础兽医学]