贵州省山羊源流产细菌调查及致病机理初探  

Preliminary Study on Abortion Bacteria from Goat in Guizhou Province and their Pathogenic Mechanism

作　　者：王伟[1] 王开功[1,2] 周碧君[1,2] 王铭[1] 冯旭芳[1] 马光强[1] 罗意[1] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州省动物疫病研究室,贵阳550025

出　　处：《中国畜牧兽医》2015年第10期2779-2787,共9页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省农业科技攻关项目"山羊繁殖障碍性疫病防控关键技术研究与示范"黔科合NY字[2011-3105]号;贵州省农业科技攻关项目"贵州省主要人畜共患病综合防控技术研究"黔科合NY字[2011]3061号;贵州省重大科技专项"酒糟资源化循环利用模式关键技术研究与集成示范"黔科合重大专项字[2011]6021-4号;贵州省农业委员会科技计划项目"贵州省2015年重大动物疫病省级定点监测"(2015-01号)

摘　　要：为调查贵州省细菌性山羊流产疫病的主要原因,分析山羊源流产细菌对正常怀孕动物免疫平衡的影响,本试验采集贵州省6个地区(州、市)12个规模养羊场临床健康和发病流产山羊的胎儿、子宫和母羊的阴道棉拭子样本共计107份进行细菌分离和鉴定,采用药敏纸片琼脂扩散法对3种主要羊源分离菌进行20种抗生素的耐药性调查,并用羊源流产细菌建立BALB/c小鼠流产模型,通过ELISA方法对妊娠相关细胞因子和激素进行测定。结果显示,从采集的107份样本中共分离到13个属细菌共302株,其中发病流产山羊生殖器官中分离的细菌种类多于临床健康山羊;流产山羊生殖器官中分离的羊源细菌耐药率高于临床健康山羊细菌耐药率;3种主要羊源流产细菌埃希氏菌、葡萄球菌和链球菌均可引起怀孕BALB/c小鼠发生不同程度的流产,流产率为埃希氏菌>葡萄球菌>链球菌;胚胎吸收率为埃希氏菌>葡萄球菌>链球菌;与空白对照组相比,各试验组BALB/c小鼠血清中的IFN-γ、IL-2含量上升,而IL-4、IL-10和孕酮含量降低。结果表明,贵州省细菌性山羊流产的发生与山羊生殖器官细菌种类多少、耐药性高低有一定相关性,推测山羊源流产细菌可能通过影响怀孕山羊的主要妊娠相关细胞因子和激素的含量导致山羊流产。In order to study the main reason of bacterial goat abortion in Guizhou province,and analyze the bacteria impact of abortion goat immune balance in normal pregnancy animals,107 samples of the goat fetus,uterus and vaginal swabs were collected from clinical health and morbidity abortion goat in 12 scale goat farms of 6areas in Guizhou province.The bacteria were isolated and identified.The drug susceptibility disc agar diffusion method was launched for the survey of the resistance of three main sources of goat isolates to 20 kinds of antibiotics.The BALB/c mice model of abortion was established using abortion bacteria isolated from goats.The pregnancy related hormones and cytokines were determined by ELISA method.The results showed that 302 strains bacteria of 13 genera were isolated from the samples,and the species of bacteria isolated from morbidity abortion goat were more than the clinical health goats.The bacteria resistant rate ofgoat reproductive organs in abortion goats was higher than that of clinical healthy goats bacteria.The varying degrees of abortion of BALB/c mice could cause by Escherichia,Staphylococcus and Streptococcus isolated from goats,the rates were Escherichia〉Staphylococcus〉Streptococcus,respectively and the embryo resorption rates were Escherichia〉Staphylococcus〉Streptococcus,respectively.Compared with the control group,IFN-γand IL-2in the serum were increased;However,IL-4,IL-10 and progesterone were decreased.The results indicated that the occurrence of bacterial goat abortion diseases had certainly related to the number of bacterial types and the level of drug resistance in goat reproductive organs.The levels of pregnancy related hormones and cytokines in goats serum could be changed by abortion bacteria,thus lead to goats abortion.

关 键 词：山羊 细菌 耐药性 细胞因子 流产 

分 类 号：S852.61[农业科学—基础兽医学]