超级细菌NDM-1质粒的水平转移研究  

Horizontal Transferability of NDM-1 Plasmid

作　　者：刘果[1] 孙洋[1] 纪雪[1] 佟盼盼[1] 祝令伟[1] 刘军[1] 周伟[1] 郭学军[1] 冯书章[1] 

机构地区：[1]军事医学科学院军事兽医研究所/吉林省人兽共患病预防与控制重点实验室,长春130122

出　　处：《中国兽药杂志》2015年第10期15-21,共7页Chinese Journal of Veterinary Drug

基　　金：科技部传染病防治重大专项(2013ZX10004217);863项目(2012AA022006);吉林省科技计划项目(20140101032JC;20150101110JC)

摘　　要：为探究超级细菌NDM-1质粒水平传播的特性,以携带NDM-1质粒的猫源洛菲不动杆菌和人源醋酸钙不动杆菌为供体菌,以工程菌株大肠杆菌DH5α和大肠杆菌、沙门氏菌弱毒菌株为受体菌,通过接合试验验证了NDM-1基因通过质粒跨种传播的可能性。结果显示,猫源NDM-1质粒可以转入E.coli DH5α中,并可以DH5α为中介,转入猪霍乱沙门氏菌C500中;而人源NDM-1质粒仅转入E.coli DH5α中,未能转入其他菌株。NDM-1质粒在沙门氏菌C500中比在E.coli DH5α中较为稳定遗传。质粒的导入并不影响宿主菌的生长特性。本研究初步揭示了NDM-1的传播方式,表明NDM-1质粒能够跨越菌种界限,并可以大肠杆菌为中介,转移至其它菌株中。The purpose of this study was to explore the horizontal transmission properties of NDM - 1 plasmids in superbugs. Acinetobacter lwoffii of cat origin and Acinetobacter calcoaceticus of human origin were used as donor strains,Escherichia coli strain DH5α and four attenuated strains（two E. coli strains and two Salmonella strains） were used as recipient strains, and conjugation experiment was conducted to explore the cross -species transferability of NDM - 1 mediated by plasmids. The results showed NDM - 1 plasmid of cat origin was able to transfer into DH5α, and then to Salmonella choleraesuis C500 strain mediated by DH5α. NDM -1 plasmid of human origin was able to transfer to DHSα, but failed to transfer to other strains. Furthermore, NDM - 1 plasmids in C500 could be more stably inherited than in E. coli DH5α. The plasmid transferred didn＇ t influence the growth properties of the host strains. This study indicated that NDM - 1 plasmids were able to cross the genetic boundaries and transfer to other strains mediated by E. coli, which may shed some light on the horizontal transmission of NDM - 1.

关 键 词：新德里β内酰胺酶-1 接合转移 水平传播 

分 类 号：S852.61[农业科学—基础兽医学]