应用去白细胞滤器加洗涤有效利用脂肪血的研究  

Chylaemia and Its Effective Reuse by Leukocytes Filtering and Washing

作　　者：明文娟 赵林园 朱琳 陈秀兰 

机构地区：[1]山东省东营市中心血站,257091

出　　处：《临床输血与检验》2015年第5期392-394,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的探讨利用现有技术合理使用已经采集的经检验合格的脂肪血。方法已经采集经检验合格的脂肪血，先进行4℃～6℃、2900g／min、10min离心，经旁路分离出脂肪血浆并热合去除，同时在此联管路上用无菌接管机接驳上四联盐水洗涤袋，逆向将相应量的生理盐水注入粘附有脂肪的红细胞保存袋内并摇匀洗涤，再关闭旁路夹进行去白过滤，离心去除洗涤生理盐水，同样方法洗涤3次，最后注入MAP红细胞保存液并留样热合，按正常去自悬浮红细胞保存35d，保存期内依次检测该产品质量，并与正常同类产品比较。结果用改进的分离洗涤方法制备的、从脂肪血分离出的红细胞，经无菌接驳生理盐水洗涤去除脂肪后，再加入MAP红细胞保存液，与非脂肪血制备的去白悬浮红细胞的质量差异无统计学意义（P〉0．05）。结论应用去白细胞滤器加洗涤处理的脂肪血，可正常应用于临床输注，减少脂肪血的浪费。Objective To study the rational utilization of chylaemia that has been tested and qualified. Methods The ehylaemia was subjected to centrifugation under 4℃ - 6℃, 2 900 g/min×10 min to remove fatty plasama to which normal saline was added,finally leukocytes were filtrated. The leukocytes of non-chylaemia was separated in routine methods with additive solution. Results No difference was seen between the erythrocytes prepared from chylaemia and those from non chylaemia in the key features of the cells for clinical applications （P〉0. 05）. Conclusion Red blood cells prepared from ehylaemia in additive solution by removing leukoeytes can be rationally used.

关 键 词：脂肪(乳糜)血 无菌接管机 红细胞添加剂 

分 类 号：R457.12[医药卫生—治疗学]