辽宁汉族人群21个非CODIS系统STR基因座的遗传多态性  

Genetic Polymorphism of 21 Non-CODIS STR Loci of Han Population in Liaoning Province

作　　者：肖南[1] 于卫建[1] 周世航[1] 

机构地区：[1]辽宁省大连市血液中心,116001

出　　处：《临床输血与检验》2015年第5期394-398,共5页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的调查辽宁汉族人群21个非CODIS系统STR基因座的遗传多态性，并对其法医学的应用价值进行评价。方法应用AGCU21＋1STR荧光检测试剂盒，对辽宁汉族207名无关个体非CODIS系统的21个STR基因座进行复合扩增，用3130基因分析仪检测扩增产物，用吻合度检验法验证样本群体的Hardy—Weinberg平衡，用PowerStats v12．xls统计分析STR基因座的群体遗传学参数。结果21个非CODIS系统STR基因座各等位基因的观察值与期望值吻合良好，符合Hardy—Weinberg平衡。杂合度（H）为0．5749～0．8213，个体识别能力（DP）为0．7904～0．9366，多态信息含量（PIC）为0．5528～0．7905，二联体非父排除率（PEduo）为0．2035～0．4657，三联体非父排除率（PEtrio）为0．3838～0．6696。二联体累积非父排除率（CPE）为0．999718，三联体累积非父排除率（CPE）为0．999999888，累积个体识别率（CDP）为0．99999999999999999995。结论21个非CODIS系统STR基因座具有良好的遗传多态性，能够有效运用于个体识别和亲子鉴定。Objective To investigate the genetic polymorphism of 21 non-CODIS STR loci in Han population and evaluate their application of forensic identification. Methods Twenty-one non-CODIS STR loci were amplified by using an AGCU 21 ＋1 STR kit and the DNA samples were obtained from 207 unrelated individuals of Han population in Liaoning province. The amplified products were analyzed with 3130 Genetic Analyzer. The sample data were validated with the goodness of fit test method of the Hardy-Weinberg equilibrium and analyzed with PowerStats v12. xls statistical of STR loci population genetics parameters. Results Twenty-one non-CODIS STR loci were well fit to Hardy-Weinberg theoretical expectation（P〉0. 05）, which conformed to Hardy-Weinberg equilibrium. The heterozygosity（H）were 0. 574 9-0. 821 3, the discrimination power （DP） were 0. 790 4-0. 936 6, the polymorphic information content （PIC） were 0. 552 8- 0. 790 5, the probability of exclusion of duo testing （PEduo）were 0. 203 5-0. 465 7, and the probability of exclusion of trios-testing （PEtrio）were 0. 383 8-0. 669 6, respevtively. The cumulative probability of exclusion （CPE） of duo-testing was 0. 999 718, the CPE of trios-testing was 0. 999 999 888, the cumulative discrimination power （CDP） was 0. 999 999 999 999 999 999 95. Conclusion Twenty-one non-CODIS STR loci are highly polymorphic, and can be effectively applied to personal forensic identification and paternity testing.

关 键 词：法医学 短串联重复序列 非CODIS 遗传多态性 

分 类 号：Q347[生物学—遗传学] R392.11[医药卫生—免疫学]