钩端螺旋体病三种检测手段假阳性率的比较研究  

Comparison study on the false positive rate of three diagnosis methods for Leptospirosis

作　　者：汪海波[1] 赵俊华[1] 唐明慧[1] 王琪[1] 杨泽[1] 林继灿[1] 史咏梅[1] 胡孔新[2] 莫秋华[1] 

机构地区：[1]珠海国际旅行卫生保健中心,广东珠海519020 [2]中国检验检疫科学研究院,北京100123

出　　处：《热带医学杂志》2015年第9期1171-1173,共3页Journal of Tropical Medicine

基　　金："十二五"国家科技重大专项课题(2011ZX10004-001)

摘　　要：目的对钩端螺旋体病的三种不同检测手段的假阳性率进行比较研究。方法收集50例健康人的血液标本,分别采用钩体Ig G ELISA试剂盒、Ig M ELISA试剂盒和PCR方法进行检测,平行比较三种检测手段的检测结果。结果在所检测的样本中,Ig G抗体检测有4例呈阳性反应,Ig M抗体检测有11例呈阳性反应,而PCR检测全为阴性。结论由于抗体检测技术可能出现的非特异性及假阳性,有必要对钩体病进行更为特异性、更加可靠的PCR检测。Objective To compare the false positive rate of three different diagnosis methods for Leptospirosis. Methods Ig G ELISA,Ig M ELISA and PCR methods were applied to detect Leptospira in blood samples from 50 healthy individuals.Then a head-to-head comparison was conducted. Results In all those samples, four were positive for Ig G antibody while11 were positive for Ig M antibody. However, when PCR method was used, all of them showed negative results.Conclusion As the non-specific and false-positive results generated by antibody detection methods, PCR method should be used instead as a more specific and reliable diagnosis approach for Leptospira detection.

关 键 词：钩端螺旋体病 检测 ELISA PCR 方法比较 

分 类 号：R377.5[医药卫生—病原生物学]