阴道毛滴虫实用教学染色标本制作  

Trichomonas vaginalis practical teaching stained specimen making

作　　者：刘晓泉[1] 陆作洁[2] 张瑞琳[3] 石焕焕[1] 李艳文[1] 申继清[1] 田春林[1] 

机构地区：[1]广西医科大学寄生虫学教研室,广西南宁530021 [2]广西民族医院检验科,广西南宁530001 [3]中山大学中山医学院基础医学实验教学示范中心,广东广州510080

出　　处：《热带医学杂志》2015年第9期1285-1286,共2页Journal of Tropical Medicine

摘　　要：目的探索阴道毛滴虫混悬液制作薄膜的方法。方法离心沉淀收集培养的阴道毛滴虫。运用计数载玻片制作虫膜,干燥、固定、染色。结果阴道毛滴虫虫体形态特征明显,核、轴柱、前鞭毛、后鞭毛、波动膜等清晰可见。结论该染色方法制作的阴道毛滴虫染色标本质量好,虫体各部位结构清晰。Objective To explore a method for making thin film of Trichomonas vaginalis by mixed suspension. Methods Trichomonas vaginalis cells in culture were collected by centrifugal precipitation.Slide film was made by counting,drying, fixing and staining. Result The morphological characteristics of Trichomonas vaginalis-nuclear, columella,anterior flagella, posterior flagellum and fluctuations of the membrane were clearly visible. Conclusion The staining method for Trichomonas vaginalis can be used for making high quality specimens showing clear structures.

关 键 词：阴道毛滴虫 薄膜 染色 

分 类 号：R382.2[医药卫生—医学寄生虫学]