水稻窄叶突变体nl(t)的遗传分析与基因定位  

Genetic analysis and gene mapping of a narrow-leaf mutant nl(t) in rice( Oryza sativa L. )

作　　者：潘银林 毛毕刚[2] 胡远艺[2] 郭李勤 彭彦[2] 韶也 阳和华[2] 赵炳然[1,2] 

机构地区：[1]中南大学研究生院隆平分院杂交水稻国家重点实验室,长沙410125 [2]国家杂交水稻工程技术研究中心,长沙410125

出　　处：《生物学杂志》2015年第5期92-95,共4页Journal of Biology

基　　金：国家自然科学基金(31301291;31471465);国家行业科技项目(201503308)

摘　　要：研究将小粒野生稻基因组DNA通过"穗茎注射法"导入恢复系RH78后产生变异,得到突变体材料nl(t)。遗传分析表明,突变体窄叶性状是由一对隐性核基因控制的,利用分子标记将nl(t)基因定位在水稻第4号染色体长臂上RM5511和RM3843标记之间,物理距离约570 kb的范围内。为进一步研究nl(t)的功能提供参考。A narrow-leaf mutant nl（ t） used in this study was isolated from the progenies of an original mutant by injecting total DNA of Oryza minuta into restorer line RH78 （ Oryza sativa L.ssp.indica） through the spike-stalk injection method.Genetic analysis indi-cated that the narrow-leaf character of nl（ t） was controlled by a recessive nuclear gene, and the putative gene was mapped between SSR markers RM5511 and RM3843 with an approximate interval of 570 kb on the long arm of chromosome 4.These results would pro-vide significant supports for the further study of the function of the NL（ t） gene.

关 键 词：水稻 窄叶 基因定位 穗茎注射法 

分 类 号：Q754[生物学—分子生物学] S511[农业科学—作物学]