蒿属香豆素对胰腺星状细胞的氧化、活化及纤维化的影响  

Influence of scoparone on oxidation,activation and fibrosis of pancreatic stellate cells

作　　者：张尤历[1] 蔡菁[1] 赵义[1] 陈晖[1] 王国英[1] 王国乙 李萍[1] 周冰洁[1] 翁星月 徐岷[1] 

机构地区：[1]江苏大学附属医院消化内科,镇江212001

出　　处：《中华中医药杂志》2015年第10期3697-3700,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省自然科学基金(No.BK20131247);镇江市"169四期工程"科研项目~~

摘　　要：目的:探讨中药茵陈蒿的主要成分蒿属香豆素对大鼠胰腺星状细胞(PSCs)氧化、活化及细胞外基质沉积的影响。方法:分离PSCs并培养,免疫荧光法鉴定细胞,培养并传代至第3-5代后进行实验,分成空白组、丹酚酸B组、不同浓度香豆素组。分别培养24、48h后MTT法检测PSCs增殖情况。48h后通过比色法检测各组细胞丙二醛(MDA)、超氧化物歧化酶(SOD)水平的变化。免疫荧光、Western blot法检测各组平滑肌肌动蛋白(α-Smooth muscle actin,α-SMA)、I型胶原表达的情况。结果:与空白组相比,蒿属香豆素组的细胞增殖水平、MDA水平、α-SMA、Ⅰ型胶原的表达明显降低,SOD水平显著增高(P<0.05)。结论:蒿属香豆素能有效降低PSCs的氧化应激水平,抑制其活化进而减轻纤维化。Objective：To investigate the influence of scoparone on oxidation,activation and fibrosis of pancreatic stellate cells and deposition of extracellular matrix.Methods：PSCs were separated and cultivated.PSCs were identified by immunofluorescence method.The cells could be experimented when cultured in 3-5 generations.PSCs were divided into blank control group,positive control group and different concentrations of scoparone groups.After 24 h and 48 h,the cells proliferation was measured by MTT assay.The levels of intracellular malondialdehyde（MDA） and superoxide dismutase（SOD） were detected by colorimetric method after 48 h.The expression of α-Smooth muscle actin（α-SMA） and Collagen α were identified by immunofluorescence and Western blot.Results：Compared with blank control group,the levels of cells proliferation and intracellular MDA,and also the expression of α-SMA and Collagen：in the experimental groups decreased significantly,while the levels of SOD increased（P〈0.05）.Conclusion：Scoparone could reduce the level of oxidative stress,suppress the activation of PSCs and alleviate fibrosis.

关 键 词：蒿属香豆素 氧化应激 胰腺星状细胞 纤维化 

分 类 号：R285.5[医药卫生—中药学]