推拿手法对家兔失神经支配后骨骼肌IGF-Ⅰ、bFGF、MyoD表达的影响  

Influence of tuina manipulation on expression of IGF-I,bFGF and MyoD in skeletal muscle after denervation of rabbits

作　　者：翁军[1] 郭汝宝[1] 李增图[1] 陈远青[1] 张喜林[2] 严隽陶[2] 

机构地区：[1]浙江中医药大学附属第三医院推拿科,杭州310005 [2]上海中医药大学附属岳阳中西医结合医院推拿科,上海200437

出　　处：《中华中医药杂志》2015年第10期3716-3719,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30973806,No.81303064);国家临床重点专科建设项目(No.ZK1101TN008)~~

摘　　要：目的：探讨推拿手法延缓周围神经损伤后失神经肌萎缩的作用效应和途径。方法：将实验家兔90只随机分为手法治疗组42只、模型对照组42只和正常组6只,手法治疗组给予按揉法治疗,模型对照组与正常组不做手法处理。手法治疗组与模型对照组分别在造模后第1、2、3周和第1、2、4、6个月逐月各取1小组（6只家兔）检测胰岛素样生长因子-I（IGF-I）、碱性成纤维细胞生长因子（b FGF）、生肌决定因子（Myo D）的表达。结果：手法治疗组在1、2周IGF-I m RNA相对表达量高于模型对照组,手法治疗组在1周、2月、4月b FGF m RNA相对表达量低于模型对照组,手法治疗组在1周、1月Myo D m RNA相对表达量高于模型对照组,在3周、4月Myo D m RNA相对表达量低于模型对照组,差异均有统计学意义（P〈0.05）。结论：推拿手法通过干预骨骼肌内IGF-I、b FGF、Myo D的表达来促进骨骼肌再生修复。Objective：To investigate the effects and action ways of tuina manipulation onamyotrophy after denervation caused by peripheral nerve injury.Methods：Ninety rabbits were randomly divided into control group（6 cases）,model group（42 cases） and tuina manipulation treatment group（42 cases）.Rabbits in tuina manipulation treatment group received tuina manipulation therapy,and rabbits in control group and model group didn＇t receive any processing.The mRNA expressions of IGF-I,bFGF and MyoD in rabbits of treatment and model groups were tested in the 1 st,2nd,3rd week,and the 1 st,2nd,4th and6 th month after model establishing.Results：The mRNA expression of IGF-I in rabbits of treatment group was higher than that of rabbits in model group in the 1 st and 2nd week（P〈0.05）.The mRNA expression of bFGF in rabbits of treatment group was lower than that of rabbits in model group in the 1 st week,and the 2nd and 4th month（P〈0.05）.The mRNA expression of MyoD in rabbits of treatment group was lower than that of rabbits in model group in the 3rd week and the 4th month,which was higher than that of rabbits in model group in the 1 st week and the 1 st month（P〈0.05）.Conclusion：Tuina manipulation could promote the regeneration and repair of skeletal muscle by regulating the expression of IGF-I,bFGF and MyoD in skeletal muscle.

关 键 词：失神经支配 成肌相关因子 推拿手法 骨骼肌萎缩 胰岛素样生长因子-I 碱性成纤维细胞生长因子 生肌决定因子 

分 类 号：R244.1[医药卫生—针灸推拿学]