祛脂愈肝颗粒对非酒精性脂肪性肝炎模型大鼠肿瘤坏死因子-α、瘦素的影响  

Effects of Quzhiyugan Granules on the expression of TNF-α and leptin in the non-alcoholic steatohepatitis rats

作　　者：赵琦[1] 何鲜平[1] 游绍伟[1] 杨梅[1] 杨玉涛[1] 

机构地区：[1]贵阳中医学院第二附属医院消化内科,贵阳550003

出　　处：《中华中医药杂志》2015年第10期3723-3725,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：贵州省科技厅资助项目(No.[2012]LKZ7017)~~

摘　　要：目的:探讨肿瘤坏死因子-α(TNF-α)及瘦素对非酒精性脂肪性肝炎(NASH)病理学影响及祛脂愈肝颗粒干预作用。方法:60只SD大鼠采用随机数字表法分为空白组、模型组、对照组和祛脂愈肝颗粒高、中、低剂量组,每组10只。空白组给予普通饲料,其余均采用高脂饲料建立大鼠NASH模型。造模6周后,对照组给予水飞蓟宾灌胃;祛脂愈肝颗粒高、中、低剂量组分别给予祛脂愈肝颗粒高、中、低剂量干预治疗。8周后测定各组谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆固醇(TC)、甘油三酯(TG)及TNF-α、瘦素水平,肝组织切片行HE染色,观察肝组织病理变化。结果:与空白组比较,模型组血清ALT、AST、TG、TC、TNF-α及瘦素均升高(P<0.01)。与模型组比较,祛脂愈肝颗粒高、中、低剂量组血清ALT、AST、TG、TC、TNF-α及瘦素均下降(P<0.01,P<0.05),祛脂愈肝颗粒中、高剂量组与对照组比较,血清ALT、AST、TG、TC、TNF-α及瘦素均下降(P<0.01,P<0.05)。结论:祛脂愈肝颗粒可能通过降低TNF-α、瘦素水平,减轻肝脏炎性反应,改善肝细胞脂肪沉积,且以祛脂愈肝颗粒高剂量效果最优。Objective：To probe how TNF-α and leptin influence the non-alcoholic steatohepatitis（NASH） in the pathologies,and the intervention effect of using Quzhiyugan Granules to treat it.Methods：We devided 60 SD rats into six groups（blank group,model group,control group,high dose Quzhiyugan Granules group,middle dose Quzhiyugan Granules group,low dose Quzhiyugan Granules group）（n=10）.Using standard diet in blank group,the other groups had high fat diet to build the NASH models.After 6 weeks,the control group was administrated silybin into stomach,the other treatment groups were respectively administrated high,middle,low dose Quzhiyugan Granules group to intervene.8weeks later,we measured the level of ALT,AST,TC,TG,TNF-α and leptin in every group,dyeing hepatic tissue section with HE-staining to observe the pathological change in it.Results：We concluded that compared to the blank group,there was a higher level of ALT,AST,TG,TC,TNF-αand leptin in all the model groups（P〈0.01）.Comparing with the model group,the treatment group had a higher level of ALT,AST,TG,TC,TNF-α and leptin（P〈0.01,P〈0.05）,and with the control group,a higher level of ALT,AST,TG,TC,TNF-αand leptin had expressed in the high dose Quzhiyugan Granules group and middle dose Quzhiyugan Granules group（P〈0.01,P〈0.05）.Conclusion：It＇s suggested that through reducing the level of TNF-α and leptin,Quzhiyugan Granules could alleviate the inflammation of liver,improve hepatocyte fatty deposits,by the way,the high dose has the optimal effect.

关 键 词：祛脂愈肝颗粒 非酒精性脂肪性肝炎 肿瘤坏死因子-Α 瘦素 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]