基于蕨素聚类分析的凤尾蕨属药用植物化学分类研究  

Study on the Chemotaxonomy of Genus Pteris by Cluster Analysis of Pterosins

作　　者：刘建群[1] 刘健[1] 

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《中国野生植物资源》2015年第5期4-8,27,共6页Chinese Wild Plant Resources

基　　金：国家自然科学基金资助项目(30960495)

摘　　要：目的:研究凤尾蕨属5种药用植物蕨素类活性成分,为其质量评价、鉴定及亲缘关系研究提供依据,为凤尾蕨属植物化学分类深入研究奠定基础。方法:采用HPLC-DAD测定5种药用植物的石油醚、乙酸乙酯、正丁醇和水部位的蕨素类成分。比较蕨素种类和含量差异,并以蕨素类特征成分及其含量为分类性状,采用聚类分析对凤尾蕨属药用植物进行化学分类研究。结果:从大叶井口边草、半边旗、全缘凤尾蕨、蜈蚣草和凤尾草中分别检测出蕨素类成分59、39、3、7和22个,总体来看大叶井口边中蕨素类成分含量最高,而全缘凤尾蕨中蕨素类成分含量最低。运用系统聚类将5种药用植物聚成3类:第Ⅰ类为全缘凤尾蕨、蜈蚣草和凤尾草;第Ⅱ类为半边旗;第Ⅲ类为大叶井口边草。结论:半边旗与全缘凤尾蕨、蜈蚣草和凤尾草之间的化学亲缘关系较远。蕨素聚类结果与传统分类结果有较好的一致性。蕨素类成分HPLC-DAD图谱可用于凤尾蕨属植物的鉴别和分类研究,并可为其质量评价提供重要参考。Objective： To study on the chemotaxonomy of Pteris by cluster analysis of pterosins. Methods：The HPLC-DAD method was established for the determination of the pterosins in five Pteris species. A cluster analysis of the characteristic pterosins was used for the chemotaxonomy of these Pteris species. Results： 59,39,3,7 and 22 pterosins were identified by HPLC-DAD from Pteris cretica L. var. nervosa,Pteris semipinnata,Pteris insignis,Pteris vittata and Pteris multifida,respectively. Pteris cretica L. var.nervosa showed the highest amount of pterosins and Pteris insignis showed the lowest content of pterosins in five species. A cluster analysis of these species of Pteris indicated that they were divided into three groups： Pteris insignis,Pteris vittata and Pteris multifida were classified into group Ⅰ,Pteris semipinnata in group Ⅱ,Pteris cretica L. var. nervosa in group Ⅲ,respectively. Conclusion： The significant differences between Pteris insignis,Pteris vittata,Pteris multifida with Pteris semipinnata were observed. The obtained results were in agreement with the traditional taxonomic study. The result indicted that the above method can be used to authenticate,classify and quality control Pteris species.

关 键 词：凤尾蕨属 蕨素 高效液相色谱法 聚类分析 化学分类 

分 类 号：R284.1[医药卫生—中药学]