花背蟾蜍皮肤结构及其抗氧化和免疫功能的研究  

Microstructure of toad(Bufo raddei)andits antioxidant and immune function

作　　者：高凤娟[1] 李丕鹏[1] 陆宇燕[1] 

机构地区：[1]沈阳师范大学两栖爬行动物研究所,沈阳110034

出　　处：《沈阳师范大学学报（自然科学版）》2015年第3期324-328,共5页Journal of Shenyang Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30870276)

摘　　要：为了深入了解花背蟾蜍(Bufo raddei Strauch)皮肤显微结构及其相关功能,利用石蜡切片及酶联免疫吸附反应技术,观察了花背蟾蜍背腹侧皮肤及其皮肤腺的显微结构特点,分别测定了背、腹侧皮肤中SOD、CAT、MDA、TLR4和βD-1含量。结果:花背蟾蜍皮肤结构与其他两栖类皮肤结构基本相同,其角质层腹侧较背侧厚,表皮和真皮背侧较腹侧厚;颗粒腺主要存在于背侧,而黏液腺主要存在于腹侧。SOD和TLR4含量腹侧皮肤高于背侧皮肤;CAT含量背侧皮肤较腹侧高;MDA和βD-1含量在背腹皮肤中相近。结论:花背蟾蜍皮肤结构和相关蛋白水平能够较好的适应其生境,并具有一定的抗氧化能力和天然免疫功能。In order to understand the skin structure and function of the toad（Bufo raddei）,the conventional paraffin section technique and ELISA were adopted to observe the microstructural characteristics of its dorsal skin,ventral skin and skin glands;and determine the contents of SOD,CAT,MDA,TLR4andβD-1of the dorsal skin and ventral skin,respectively.The results show that the toad skin structures are similar to other amphibians.That is the ventral cuticle thicker than the dorsal,the dorsal epidermis and dermis thicker than the ventral,granular glands mainly in the dorsal skin,and mucous glands in the ventral skin.SOD and TLR4 examined in the ventral skin are higher than in the dorsal skin,CAT containing in the dorsal skin is higher than in the ventral skin,but MDA andβD-1are similar in these two areas.In conclusion,the structures and related protein levels of the toad skin closely adapt to the living environment,and have the antioxidant capacities and natural immune functions.

关 键 词：花背蟾蜍 皮肤 显微结构 SOD TLR4 βD-1 

分 类 号：Q246[生物学—细胞生物学] Q136