白血病细胞中Beta-Arrestin1与EZH2分子结合研究  

Analysis of the interaction of Beta-Arrestin1 and EZH2 in leukemia cells

作　　者：王毅[1] 舒逸[2] 秦茹[2] 陈卉[2] 苑俊涛 邹琳[2] 

机构地区：[1]重庆医科大学附属儿童医院临床分子医学中,心儿童发育疾病研究教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地 [2]重庆医科大学附属儿童医院临床分子医学中心,儿童发育疾病研究教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地

出　　处：《重庆医科大学学报》2015年第9期1215-1218,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金面上资助项目(编号:81373444)

摘　　要：目的:检测急性淋巴细胞白血病细胞(acute lymphoblastic leukemia,ALL)CCRF-CEM和Raji细胞中Beta-抑制蛋白1(Beta-Arrestin1)与Zeste增强子同源物-2蛋白(enhancer of Zeste homolog 2,EZH2)是否存在结合。方法:白血病细胞中,Western blot检测Beta-Arrestin1与EZH2表达水平,激光共聚焦(Confocal)检测Beta-Arrestin1与EZH2在细胞中的位置与共定位;免疫共沉淀(CO-IP)检测Beta-Arrestin1与EZH2结合能力。结果:Beta-Arrestin1与EZH2在白血病K562、CCRF-CEM和Raji细胞中均有表达。激光共聚焦结果显示Beta-Arrestin1与EZH2均在K562、CCRF-CEM和Raji细胞内共定位,CO-IP结果显示在K562,CCRF-CEM和Raji细胞中Beta-Arrestin1与EZH2结合。结论:在CCRF-CEM和Raji中,Beta-Arrestin1可与EZH2结合。Objective：To test that if Beta-Arrestinl could bind with enhancer of Zeste homolog 2（EZH2） in acute lymphoblastic leukemia（ALL） CCRF-CEM and Raji cells. Methods：The protein levels of Beta-Arrestinl and EZH2 in the leukemia ceils were determined by Western blot. The location of Beta-Arrestinl and EZH2 in the leukemia cells was measured by confocal microscopy. Co-immunoprecipitation（CO-IP） was examined for the binding of Beta-Arrestinl with EZH2 in leukemia cells. Results：Western blot results showed that b/Beta-Arrestinl and EZH2 expressed in those three leukemia cells. Confocal data showed the colocaliza- tion of Beta-Arrestinl and EZH2 in K562, CCRF-CEM and Raji cells. CO-IP assay illustrated that Beta-Arrestinl bind with EZH2 in three leukemia cells. Conclusion：Beta-Arrestin 1 could bind to EZH2 in ALL CCRF-CEM and Raji cells.

关 键 词：白血病 Beta-抑制蛋白1 Zeste增强子同源物-2蛋白 蛋白结合 

分 类 号：R34[医药卫生—基础医学]