超高效液相色谱-串联质谱法测定莲雾中多菌灵、噻菌灵和甲霜灵残留  

Determination of carbendazim,thiabendazole and metalaxyl residues in wax-apple by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：李春丽[1] 乐渊[1] 尹桂豪[1] 阳辛凤[1] 黎舒怀[1] 刘春华[1] 

机构地区：[1]中国热带农业科学院分析测试中心,海口571101

出　　处：《农药学学报》2015年第5期579-584,共6页Chinese Journal of Pesticide Science

摘　　要：建立了莲雾中多菌灵、噻菌灵和甲霜灵残留的超高效液相色谱-串联质谱（UPLC-MS/MS）的快速检测方法。样品直接用乙腈匀浆提取,过0.22μm滤膜后,在电喷雾正离子模式下以多反应监测（MRM）模式测定,基质匹配标准溶液外标法定量。结果表明：方法的线性范围为0.01~0.5μg/m L,决定系数（R2）〉0.99;在0.04、0.4和2 mg/kg 3个添加水平下,方法的平均回收率为75%~107%,相对标准偏差为2.0%~8.9%（n=5）,定量限均为0.04 mg/kg。该方法样品前处理简单、快速,分析时间短,灵敏度、准确度和精密度均符合农药残留检测要求。A rapid method for determining carbendazim, thiabendazole and metalaxyl residue in waxapples was developed with ultra performance liquid chromatography-tandem mass spectrometry （UPLC- MS/MS） detection. The samples were extracted with acetonitrile. The samples were detected by UPLC-MS/MS under positive electrospray ionization and multiple reaction monitoring mode. The matrix-matched external standard calibration curves were used for the quantitative analysis. It was shown that the calibration curves were linear in the range of 0.01 - 0.5 μg/mL and coefficient of determination RE 〉 0.99. The limits of quantitation were 0.04 mg/kg. The average recoveries of the three pesticides were 75% -107% at spiked levels of 0.04, 0.4 and 2 mg/kg, with relative standard deviations in the range of 2.0% -8.9% （n = 5 ）. The sample pretreatment of the method is simple and time-saving. The method is applied for detection of carbendazim, thiabendazole and metalaxyl residues in market wax-aooles.

关 键 词：超高效液相色谱-串联质谱(UPLC-MS/MS) 莲雾 多菌灵 噻菌灵 甲霜灵 残留 

分 类 号：S481.8[农业科学—农药学] O657.7[农业科学—植物保护]