补肾活血方对家兔骨关节炎滑液中IL-6和MMP-13表达的影响  

Influence of Invigorating Kidney and Activating Blood Formulae on Expression of IL-6 and MMP-13 in Synovial Fluid of Rabbits with Knee Osteoarthritis

作　　者：潘贤峰 杨鑫斌 段培青 张榕[1] 肖卫国[1] 

机构地区：[1]中国医科大学附属第一医院风湿免疫科,沈阳110001

出　　处：《中国医科大学学报》2015年第10期909-912,共4页Journal of China Medical University

基　　金：辽宁省科学技术计划项目(2012225097)

摘　　要：目的探讨补肾活血方对家兔骨关节炎滑液中基质金属蛋白酶13(MMP-13)和白介素6(IL-6)表达的影响,推断补肾活血方治疗骨关节炎的作用机制。方法选取新西兰家兔30只以改良型Hulth方法建立膝骨关节炎模型,随机分为空白组、模型组、补肾组、活血组和补肾活血组。分别给予对应的自拟汤剂,模型组给予生理盐水,在喂养后的第4周、第8周和第12周分批次提取膝关节滑液。应用ELISA法检测家兔膝骨关节滑液中MMP-13和IL-6的含量。结果家兔膝骨关节炎关节滑液中IL-6水平明显高于正常对照组,尤其第4周、第8周升高最为明显(P<0.001),而第12周时IL-6与对照组比较无统计学差异;另外MMP-13水平在第4周、第8周及第12周均明显高于空白对照组(P<0.001)。给予自拟中药汤剂喂养第8周后补肾组、活血组、补肾活血组IL-6水平均明显下降,与模型组比较有统计学差异(P<0.001),但是在第12周时各组间无统计学差异,此外喂养第12周后3组的MMP-13水平也明显低于模型组(P<0.001)。结论 IL-6和MMP-13可能参与家兔膝骨关节炎发生发展。补肾活血方可以减少IL-6及MMP-13的表达,可能减轻骨关节炎的进展,并且优于单纯补肾方剂或活血方剂。Objective To investigate the expression levels of Interleukin（IL）- 6 and matrix metalloproteinases（MMP）-13 in synovial fluid by invigorating kidney and activating blood formulae in treating rabbit knee osteoarthritis model. Methods A total of 30 New Zealand rabbit were randomly divided into blank group,model group,invigorating kidney group,activating blood group and invigorating kidney and activating blood group.Rabbits model with knee osteoarthritis were established by improved Hulth method. To give corresponding respectively the medicinal broth,model group was given saline,knee joint synovial fluid was collected after 4,8 and 12 weeks. Enzyme linked immunosorbent assay（ELISA）was used to measure the levels of IL-6 and MMP-13. Results The levels of IL- 6 in rabbit knee osteoarthritis were obviously higher than that of normal control group at both 4 weeks and 8 weeks（P〈0.001）. But there was no statistical difference on the levels of IL- 6 compared with controls in 12 weeks. In addition,the level of MMP- 13 at 4 weeks,8 weeks and 12 weeks were significantly higher than the blank control group（P〈0.001）. After 8 weeks of Chinese medicine administration,the levels of IL- 6 in synovial fluid were significantly decreased in invigorating kidney group,activating blood group and invigorating kidney and activating blood group（P〈0.001）,but there was no statistical difference among groups in 12 weeks. The MMP-13 levels of synovial fluid was significantly lower than the model group（P〈0.001）. Conclusion Our results indicate that IL- 6 and MMP- 13 participate in the pathological development of the rabbit knee osteoarthritis. Invigorating kidney and activating blood formulae could reduce the expression of IL- 6 and MMP- 13 and alleviate osteoarthritis progression,and which is superior to the pure invigorating kidney formulae and activating blood formulae.knee osteoarthritisIL-6MMP-13 invigorating kidney and activating blood formulae

关 键 词：膝骨关节炎 IL-6 MMP-13 补肾活血方 

分 类 号：R684.3[医药卫生—骨科学]