深海来源链霉菌12A35产生新抗生素lobophorin J的分离和鉴定  

Isolation and identification of a new antibiotic, lobophorin J, from a deep sea-derived Streptomyces sp.12A35

作　　者：宋春凤 潘华奇[1] 胡江春[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016 [2]中国科学院大学,北京100049

出　　处：《中国抗生素杂志》2015年第10期721-727,784,共8页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.41006088;No.41576136);国家科技支撑计划课题(No.2011BAE06B04);国家高技术研究发展计划(863计划)课题(No.2012AA092104;No.2011AA09070404);辽宁省科技计划项目(2014214004)

摘　　要：目的从分离自中国南海深海沉积物的链霉菌12A35中分离和鉴定lobophorins类抗菌活性产物。方法采用活性导向的分离方法，利用大孔吸附树脂、正相硅胶柱色谱、反相ODS柱色谱和半制备高效液相色谱进行活性产物的分离纯化；使用。D—NMR和HR-ESI—MS进行结构鉴定。结果分离和鉴定了一个新的spirotetronates家族抗生素，命名为lobophorinJ。结论深海链霉菌12A35产生新的抗生素lobophorinJ。Objective To isolate and identify antibacterial active lobophorins from Streptomyces sp. 12A35 which was isolated from a deep-sea sediment collected in the South China Sea. Methods Bioassay-guided fractionation was extracted with various chromatographic methods, including HP20 adsorption, flash chromatography over silica gel, octadecylsilyl （ODS） and semi-preparative HPLC. Its structure was elucidated based on the spectroscopy of HR-ESI-MS and 1D-NMR. Results A new spirotetronate antibiotics was isolated and determined to be lobophorin J. Conclusion The deep sea-derived Streptomyces sp. 12A35 produced lobophorin J as a new antibiotic.

关 键 词：链霉菌属 海洋放线菌 特窗酸类螺环化合物:Lobophorins抗菌活性 

分 类 号：R978.19[医药卫生—药品]