HPLC法测定芪黄胶囊中青藤碱的含量  

Content Determination of Sinomenine in Qihuang Capsule by HPLC

作　　者：阳勇[1] 罗维早[1] 梁旭明[1] 秦伟瀚[1] 卿大双 王德江[1,2] 王颖[3] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]成都中医药大学药学院,成都610072 [3]重庆市中医院药剂科,重庆400021

出　　处：《中国药房》2015年第30期4243-4244,共2页China Pharmacy

基　　金："重大新药创制"科技重大专项(No.2013ZX09101108);重庆市卫生局中医药科技项目(No.ZY20132071)

摘　　要：目的：建立测定芪黄胶囊中青藤碱含量的方法。方法：采用高效液相色谱法。色谱柱为Welch Ultimate AQ-C18,流动相为甲醇-磷酸盐缓冲液（梯度洗脱）,流速为1.0 ml/min,检测波长为264 nm,柱温为30℃,进样量为10μl。结果：青藤碱检测进样量线性范围为0.200 3～10.016 0μg（r=0.999 8）;精密度、稳定性、重复性试验的RSD〈1.0%;加样回收率为98.80%～100.94%,RSD为0.79%（n=6）。结论：该方法简便、准确、重复性好,可用于芪黄胶囊中青藤碱的含量测定。OBJECTIVE： To establish a method for the content determination of sinomenine in Qihuang capsule. METHODS： HPLC was performed on the column of welch C18-AQ with mobile phase of methanol-phosphate buffer（gradient elution） at flow rate of 1.0 ml/min, detection wavelength was 264 nm, column temperature was 30℃ and volume injection was 10 μl. RESULTS： The linear range of sinomenine was 0.200 3-10.016 0 μg（r=0.999 8）, RSDs of precision, stability and reproducibility tests were lower than 1.0%, recovery was 98.80%-100.94% （RSD=0.79% ,n=6）. CONCLUSIONS： The method is simple, accurate and re- producible, and can be used for the content determination of sinomenine in Qihuang capsule.

关 键 词：芪黄胶囊 青藤碱 含量 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]