双波长HPLC法同时测定滋补肝肾丸中4种成分的含量  

Simultaneous Determination of the Contents of 4 Ingredients in Zibu Ganshen Pill by Dual-wavelength HPLC

作　　者：王玉娟[1] 陶利[1] 崔苏镇[1] 梁竹[1] 周金辉[2] 

机构地区：[1]济南军区总医院药剂科,济南250031 [2]济宁医学院药学院,山东日照276000

出　　处：《中国药房》2015年第30期4266-4268,共3页China Pharmacy

摘　　要：目的：建立同时测定滋补肝肾丸中女贞苷、特女贞苷、异去甲蟛蜞菊内酯和蟛蜞菊内酯含量的方法。方法：采用双波长高效液相色谱法。色谱柱为Elite C18,流动相为乙腈-0.5%醋酸溶液（梯度洗脱）,流速为0.9 ml/min,柱温为25℃,检测波长0～30min为224 nm、30～50 min为351 nm,进样量为20μl。结果：女贞苷、特女贞苷、异去甲蟛蜞菊内酯和蟛蜞菊内酯质量浓度分别在6.75～135.00、6.54～130.80、4.90～98.00、6.42～128.40μg/ml范围内与各自峰面积呈良好的线性关系（r=0.999 5、0.999 8、0.999 4、0.999 6）;精密度、稳定性、重复性试验的RSD≤1.25%;加样回收率为96.15%～99.96%,RSD〈2%（n=6）。结论：该方法快速、灵敏、准确,可用于滋补肝肾丸中女贞苷、特女贞苷、异去甲蟛蜞菊内酯和蟛蜞菊内酯的含量测定。OBJECTIVE： To establish a method for simultaneous determination of the contents of ligustroflavone, specnuezhe- nide, demethylwedelolactone and wedelolactone in Zibu ganshen pill. METHODS：Dual-wavelength HPLC was performed on the column of Elite C18 with mobile phase of acetonitrile-0.5% acetic acid （gradient elution） at flow rate of 0.9 ml/min, column temper- ature was 25 ℃, detection wavelengths were 224 nm（0-30 min） and 351 nm （30-50 min）, and the injection volume was 20 μl. RESULTS： The linear range was 6.75-135.00 μg/ml（r=0.999 5） for ligustroflavone, 6.54-130.80 μg/ml（r=0.999 8） for specnuezhe- nide,4.90-98.00 μg/ml（r=0.999 4） for demethylwedelolactone and 6.42-128.40 μg/ml（r=0.999 6） for wedelolactone; RSDs of pre- cision, stability and reproducibility tests were no more than 1.25% ;average recoveries were 96.15%-99.96% （RSD〈2%, n=6）. CONCLUSIONS： The method is rapid, sensitive and accurate, and can be used for the contents determination of ligustroflavone, specnuezhenide, demethylwedelolactone and wedelolactone in Zibu ganshen pill.

关 键 词：滋补肝肾丸 女贞苷 特女贞苷 异去甲蟛蜞菊内酯 蟛蜞菊内酯 双波长高效液相色谱法 

分 类 号：R446.1[医药卫生—诊断学]