基于脯氨酸仿生合成的纳米TiO_2吸附牛血清白蛋白行为的研究  

The adsorption behavior of Bovine Serum Albumin onto Nano-TiO_2 biomimetic synthesis based on proline and bovine serum albumin

作　　者：宫建龙[1] 杨良军[1] 朱龙宝[1] 刘任龙[1] 陶玉贵[1] 梁敏东 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《安徽工程大学学报》2015年第4期7-11,共5页Journal of Anhui Polytechnic University

基　　金：安徽省自然科学基金资助项目(1308085MC51);安徽省高校自然科学基金资助项目(KJ2012A034);安徽省高校青年科研基金资助项目(2003JQL370);国家级大学生创新创业训练计划基金资助项目(201410363023)

摘　　要：以基于脯氨酸仿生合成的纳米TiO2为吸附剂,研究其对牛血清白蛋白(BSA)的吸附行为,并采用傅立叶变换红外光谱(FT-IR)以及差热-热重分析(DTA-TG)对其界面进行表征.研究结果表明:吸附等温线符合Langmuir吸附模型,为单分子层吸附,最大吸附量为40.1mg/g,吸附常数为5.71mL/mg;吸附过程可用Lagergren拟一级动力学方程描述;吸附过程为放热物理吸附.界面表征纳米TiO2与BSA间吸附作用力为氢键和范德华力,与拟合曲线的分析结果相吻合.The adsorption behavior of bovine serum albumin（BSA）onto Nano-TiO2 biomimetic synthesis based on Proline was investigated.Fourier transform infrared spectrophotometry（FT-IR）and Differential Thermal Analysis-Thermal Gravimetry（DTA-TG）were employed to interface characterization.The results showed that fitting curve of isotherm adsorption accorded with Langmuir adsorption model,and the adsorption was monomolecular layer adsorption,and the maximum adsorption capacity was40.1mg/g and adsorption constant was 5.71mL/mg.the adsorption process can be described by Lagergren pseudo-first-order kinetic model,belonging to an exothermic physical adsorption.Interface characterization showed that the interaction between Nano-TiO2 and BSA was hydrogen bond and Vander Waals which conforms to the results of analysis of fitting curves.

关 键 词：纳米二氧化钛 牛血清白蛋白 吸附行为 吸附动力学 

分 类 号：O614.41[理学—无机化学]