精液白细胞异常增加对精子DNA氧化损伤的影响  

Impacts of leukocyte elevation on reactive oxygen species and sperm DNA damage

作　　者：张俏忻[1] 肖颖秀[2] 程碧珍[1] 

机构地区：[1]汕头大学医学院第一附属医院检验科,广东汕头515041 [2]汕头大学医学院第一附属医院神经内科,广东汕头515041

出　　处：《癌变．畸变．突变》2015年第5期383-385,393,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省医学科研基金资助项目(A2014441)

摘　　要：目的:研究精液白细胞异常增加对精液活性氧(ROS)及精子DNA损伤的影响。方法:共收集52例精液白细胞异常增高标本和50例白细胞正常标本,以鲁米诺为探针,运用化学发光法检测精子活性氧水平。采用精子染色质结构分析(SCSA)和单细胞凝胶电泳(SCGE)分析精子核DNA损伤。结果:白细胞异常组较白细胞正常组精液标本中ROS显著增加(P<0.01),SCSA结果显示白细胞异常组DNA碎片指数(DFI)、高DNA可染性(HDS)较白细胞正常组显著增加(P=0.020,P=0.012);SCGE结果显示白细胞异常组彗星尾矩(TM)较白细胞正常组显著增加(P=0.007)。结论:精液白细胞异常升高可引起ROS增加,造成精子核DNA的断裂损伤。OBJECTIVE： To study the impacts of leukocyte elevation on reactive oxygen species （ROS） and sperm DNA damage. METHODS： A total of 52 elevated leukocyte specimen and 50 normal leukocytes specimen were collected. ROS production was measured using luminol as the probe by the Chemiluminescence method. Sperm nuclear DNA damage was assessed by sperm chromatin structure assay （SCSA） and single-cell gel electrophoresis （SCGE）. RESULTS： ROS levels （using the log-transformation） was significantly higher in the elevated leukocyte group （P〈0.01）. Data of SCSA indicated there were significant increases of DNA fragmentation index and high DNA stainable in the elevated leukocytes group （P=0.020, P=0.012）. SCGE analysis, in terms of tail moments, was significantly higher in the elevated leukocyte group （P=0.007）. CONCLUSION： Leukocyte elevation could result in the increase of reactive oxygen species, which may induce sperm nuclear DNA damage.

关 键 词：白细胞 精子 活性氧 DNA损伤 

分 类 号：R698.2[医药卫生—泌尿科学]