海洋拟诺卡菌SCSIO 11492中次生代谢产物的分离及其抗肿瘤活性研究  

Sepration and antitumor activities of secondary metabolites from marine actinomycete Nocardiopsis sp.SCSIO 11492

作　　者：苏瑞强[1] 李晏 彭坤[1] 李洁[3] 杨全[1] 杨献文[3] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]解放军413医院器械科,浙江舟山510301 [3]中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室,广东广州510301

出　　处：《药学实践杂志》2015年第5期406-410,共5页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金(21002110;21372233)

摘　　要：目的探索海洋来源的拟诺卡菌(Nocardiopsis sp.)SCSIO 11492的抗肿瘤活性次生代谢产物。方法采用硅胶、凝胶、ODS等色谱分离方法对海洋来源的拟诺卡菌SCSIO 11492的发酵粗提物进行分离、纯化,根据次生代谢产物的核磁数据及相关的理化性质确定化合物的结构,最后利用活性测试发现活性成分。结果从海洋拟诺卡菌SCSIO 11492中分离纯化得到5个化合物,经鉴定分别为2′-脱氧腺苷(2′-deoxyadenosine,1)、2′-脱氧胸腺嘧啶核苷(2′-deoxythymidine,2)、2′-脱氧尿苷(2′-deoxyuidine,3)、尿嘧啶核苷(uridine,4)、1-O-棕榈酰基-3-O-β-D-半乳糖基甘油酯(1-O-hexadecanoyl-3-O-β-D-galactopyranosylglycerol,5)。活性测试结果表明化合物5对RAW264.7显示出较好的生长抑制活性(IC50=10.9μmol/L)。结论从海洋拟诺卡菌SCSIO 11492中分离得到5个化合物,均为首次从该属中分离得到。其中化合物5可能是该菌抗肿瘤的活性成分。Objective To explore cytotoxic secondary metabolites from a marine actinomycete Nocardiopsis sp .SCSIO 11492 .Methods Isolation and purification were carried out by column chromatography over silica gel ,Sephadex LH-20 ,and ODS structures of the isolates were identified mainly by NMR spectroscopic data .And cytotoxic bioassay was performed using MTT method .Results Five compounds were identified as 2′-deoxyadenosine （1） ,2′-deoxythymidine （2） ,2′-deoxyuidine （3） ,uridine （4） ,1-O-palmitoyl-3-d-galactosyl-sn-glycerol spongilipid （5） .Compound 5 exhibited weak cytotoxic activity with IC50 value of 10 .9 μmol/L .Conclusion Five compounds were obtained from a marine actinomycete Nocardiopsis sp .SCSIO 11492 .All five compounds were reported for the first time from this genus .Compound 5 could be the bioactive compound re-sponsible for the cytotoxic activity of Nocardiopsis sp .SCSIO 11492 .

关 键 词：海洋放线菌 拟诺卡菌 次生代谢产物 抗肿瘤 

分 类 号：R282.77[医药卫生—中药学]