基于整体柱的表面增强拉曼光谱法检测非法染色青黛  

Detection of malachite green in natural indigo by surface enhanced Raman spectroscopy based on monolithic column

作　　者：郑滨兴 吕迪亚 方芳[2] 刘艳华[1] 李丹[3] 陆峰[2] 许激扬[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009 [2]第二军医大学药学院,上海200433 [3]福建中医药大学药学院,福建福州350108

出　　处：《药学实践杂志》2015年第5期426-428,459,共4页Journal of Pharmaceutical Practice

摘　　要：目的建立基于整体柱的表面增强拉曼光谱(surface enhanced Raman spectroscopy,SERS)法快速检测非法染色的青黛。方法采用混合溶剂乙醇-水(1∶1,V/V)对染色青黛中的染料进行提取,利用整体柱中网状孔隙有助于银纳米颗粒在空间上耦合的特性,将样品溶液和银胶溶液混匀后滴加于整体柱上进行SERS光谱采集。结果此法可得到青黛中染色掺伪量低至500μg/kg的孔雀石绿SERS信号。结论该方法具有快速、简便、专属性强等优点,可运用于中药材青黛非法染色的快速检测。Objective To develop a rapid SERS detection method based on monolithic column for detection of dye adul-terated natural indigo .Methods The dyes in natural indigo were extracted and mixed with silver colloid .The spectra were re-corded after applying the mixture solution to the monolithic column since the intertwined pores in monolithic column could con-tribute for the distribution of silver nanoparticles .Results SERS signals of malachite green dyed natural indigo at quantity as low as 500 μg/kg could be obtained .Conclusion This simple ,fast and specific SERS detection method based on monolithic col-umn could be used for rapid detection of stained natural indigo .

关 键 词：表面增强拉曼光谱 整体柱 非法染色 青黛 孔雀石绿 

分 类 号：R927.1[医药卫生—药学]