3种不同治法的中药复方防治动脉粥样硬化的机制研究  

Prevention and Treatment of Atherosclerosis by Three Different Chinese Medical Compounds:a Mechanism Study

作　　者：姜华[1] 姜玉姬[2] 

机构地区：[1]延边大学附属医院中西医结合科,吉林延吉133000 [2]延边大学附属医院肾内科,吉林延吉133000

出　　处：《中国中西医结合杂志》2015年第10期1244-1248,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81160476)

摘　　要：目的研究补阳还五汤、血府逐瘀汤、四君子汤3种不同治法的中药复方含药血清对血管内皮细胞Toll样受体4(Toll-like receptor 4,TLR4)/NF-κB信号转导通路及下游血凝素样氧化低密度脂蛋白受体-1(lectin-like oxidized low-density lipoprotein receptor-1,LOX-1)、TNF-α、血管细胞黏附因子-1(vascular cell adhesion molecule-1,VCAM-1)及细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)等炎症因子的影响,探讨其防治动脉粥样硬化的机理。方法将20只新西兰白兔随机分为正常血清组、补阳还五汤血清组(6.7 g/kg)、血府逐瘀汤血清组(3.6 g/kg)及四君子汤血清组(1.6 g/kg),每组5只,给药7天,末次给药2 h后,心脏采血,每只约取50 mL制备药物血清。培养人脐静脉内皮细胞株ECV304 18 h后随机分为空白对照组、模型组、西药对照组、补阳还五汤组、血府逐瘀汤组、四君子汤组,除空白对照组外其他各组用LPS刺激2 h后,西药对照组及中药各组给予含10%对应含药血清干预24 h。用荧光定量PCR方法和Western blot方法测定TLR4、髓样分化因子88(myeloid differentiation factor 88,MyD88)、肿瘤坏死因子受体相关因子-6(tumor necrosis factor receptor-associated factor-6,TRAF-6)、NF-κB、LOX-1、TNF-α、VCAM-1及ICAM-1的基因及蛋白表达。结果与空白对照组比较,模型组TLR4、MyD88、TRAF-6、NF-κB、LOX-1、TNF-α、VCAM-1及ICAM-1mRNA表达升高,TLR4、NF-κB、LOX-1、TNF-α、VCAM-1及ICAM-1蛋白表达水平明显升高(P<0.01);与模型组比较,补阳还五汤组、血府逐瘀汤组及西药对照组可以抑制各指标mRNA及蛋白表达,差异有统计学意义(P<0.05);而补阳还五汤组、血府逐瘀汤组各指标mRNA表达及蛋白表达较西药对照组高,差异有统计学意义(P<0.05)。四君子汤组各项指标与其他各组比较,差异无统计学意义(P>0.05)。结论补阳还五汤和血府逐瘀汤防治动脉粥样硬化作用的机制可能与抑制TLR4/NF-κB信号�Objective To study the effect of Buyang Huanwu Decoction（BHD）,Xuefu Zhuyu Decoction（XZD）,and Sijunzi Decoction（SD） contained serums on expressions of Toll-like receptor 4（TLR4）/nuclear factor（NF）-κB signals,lectin-like oxidized low-density lipoprotein receptor-1（LOX-1）,tumor necrosis factor-α（TNF-α）,vascular cell adhesion molecule-1（VCAM-1）,intercellular adhesion molecule-1（ICAM-1）,and to explore possible anti-atherosclerotic mechanisms.Methods Twenty New Zealand rabbits were divided into 4 groups at random,i.e.,the normal control group,the BHD group（6.7g/kg）,the XZD group（3.6 g/kg）,and the SD group（1.6 g/kg）,5 in each group.All medication lasted for7 successive days.Two h after the final medication,about 50 mL blood was withdrawn from rabbit heart for preparing serums.Human umbilical vein endothelial cell ECV304 were cultured in vitro for 18 h and randomly divided into the blank control group,the model group,the Western medicine（WM） control group,the BHD group,the XZD group,and the SD group at random.ECV304,except in the blank control group,were stimulated with lipopolysaccharide（LPS） for 2 h.Those in the WM control group and CM groups were treated respectively with corresponding CM contained serum for 24 h.Finally gene and protein expressions of TLR4,myeloid differentiation factor 88（MyD88）,tumor necrosis factor receptor-associated factor-6（TRAF-6）,NF-κB,LOX-1,TNF-α,ICAM-1,and VCAM-1 were detected by fluorescent quantitative PCR and Western blot.Results Compared with the blank control group,mRNA expressions of TLR4,MyD88,TRAF-6,NF-kB,LOX-1,TNF-α,ICAM-1,and VCAM-1 increased significantly;protein expressions of TLR4,NF-kB,LOX-1,TNF-α,ICAM-1,and VCAM-1 also increased significantly in the model group（P〈0.01）.Compared with the model group,mRNA and protein expressions of each index could be significantly inhibited in the BHD group,the XZD group,and the WM control group（P〈0.05）.Besides,mRNA and protein expressions of each index could be

关 键 词：补阳还五汤 血府逐瘀汤 四君子汤 Toll样受体4 血凝素样氧化低密度脂蛋白受体-1 动脉粥样硬化 

分 类 号：R259[医药卫生—中西医结合]