灵芝菌丝体液态深层发酵合成灵芝三萜动力学研究  

Biomass Production,Carbohydrate Utilization and Triterpene Biosynthesis during Submerged Culture of Ganoderma lucidum G0119

作　　者：冯杰[1] 冯娜[1] 贾薇[1] 杨焱[1] 刘方[1] 张劲松[1] 

机构地区：[1]上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验室,上海201403

出　　处：《食用菌学报》2015年第3期74-79,共6页Acta Edulis Fungi

基　　金：上海市市级农口系统青年人才成长计划资助项目(沪农青字2015第1-7号);上海市农业科学院青年科技人员“助跑”计划(ZP06)资助

摘　　要：基于Logistic方程和Luedeking-Piret方程,对灵芝(Ganoderma lucidum)菌丝体液态深层发酵合成灵芝三萜动力学进行研究。通过检测发酵过程的菌丝体量、基质浓度和产物浓度,进行最优参数估计和非线性拟合。得到菌丝体生长、产物灵芝三萜生成和底物总糖消耗的动力学模型和模型参数。对动力学模型的拟合曲线进行分析,发酵实验值与模型预测值能较好地拟合,所建立的液态深层发酵动力学模型能较好地反应灵芝菌丝体发酵过程。Kinetic models for triterpene biosynthesis by Ganoderma lucidum mycelium grown in submerged culture were evaluated based on Logistic and Luedeking-Piret equations. Optimal parameter estimation and nonlinear fitting were completed by monitoring mycelium production, substrate concentration and product yield throughout the fermentation process, and kinetic models incorporating these parameters were proposed. Experimental and predicted values were in good agreement, and our data confirmed that the models provided a reasonable description of the fermentation process.

关 键 词：灵芝 深层发酵 动力学模型 非线性拟合 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]