内质网应激预处理对大鼠脑缺血再灌注损伤Bcl-2和Bax蛋白表达的影响  

Effect of Endoplasmic Reticulum Stress Preconditioning on Bcl-2 and Bax Expression in Rats With Cerebral Ischemic-reperfusion

作　　者：闵鹤鸣[1] 贾玉洁[2] 程雪[2] 包翠芬[1] 

机构地区：[1]辽宁医学院基础医学院细胞生物学教研室 [2]辽宁医学院附属第一医院神经内科,辽宁锦州121001

出　　处：《辽宁医学院学报》2015年第5期5-7,111,112,共5页Journal of Liaoning Medical University (LNMU) Bimonthly

基　　金：国家自然科学基金资助;编号:81202783;辽宁省自然科学基金项目;编号:20092192

摘　　要：目的探讨2-脱氧葡萄糖(2-deoxyglucose,2-DG)诱导的内质网应激(endoplasmic reticulum stress,ERS)预处理对大鼠脑缺血再灌注后神经细胞凋亡及相关蛋白Bcl-2和Bax表达的影响。方法 24只SD大鼠随机分为假手术组(SH组)、脑缺血/再灌注组(I/R组)、2-DG诱导的ERS预处理组。采用TUNEL法检测CA1区凋亡细胞,免疫组化法检测Bcl-2和Bax蛋白在海马CA1区的表达变化。结果与I/R组比较,ERS预处理组的神经行为学评分明显降低、凋亡细胞数目亦明显减少(P<0.05);Bcl-2蛋白表达细胞数明显增加(P<0.01),Bax蛋白表达的细胞数明显减少(P<0.01)。结论 ERS预处理对脑缺血再灌注损伤具有保护作用,其机制可能与其影响Bcl-2和Bax蛋白表达有关。Objective To explore the effect of preconditioning the endoplasmic reticulum stress induced by 2 - DG on the neural cell apoptosis and the expression of related proteins, Bel -2 and Bax, in the rats with cerebral ischemic - reperfusion. Method 24 SD rats were divided into 3 groups on average： the sham -operation group （SH group）, ischemic -reperfusion group （I/R group）, and endoplasmic reticulum stress （ induced by 2 - DG） preconditioning group （ ERS induced by 2 - DG group）. The apoptotic cells in the hippocampal field CAI were tested with TUNEL, and the Bcl -2 and Bax protein expression changes in the same field were detec- ted with the immunohistochemical method. Result The neurobehavioral scores and the apoptosis cell number significantly decreased in the ERS preconditioning group compared with those in the I/R group （ P 〈 0. 05 ）. The cell number of Bel - 2 protein expression signif- icantly increased （ P 〈 0. 01 ）, while the cells with Bax protein expression significantly decreased （ P 〈 0. 01 ）. Conclusion Endoplas- mic reticulum stress preconditioning provides protection for cerebral ischemic - reperfusion injury by exerting its effect on Bcl - 2 and Bax protein expression.

关 键 词：内质网应激 预处理 缺血再灌注 BCL-2 BAX 

分 类 号：R338[医药卫生—人体生理学]