硫化氢抗哇巴因诱发大鼠触发性心律失常作用的观察  

Effects and Mechanism of Hydrogen Sulfide on Ouabain-induced Arrhythmias

作　　者：刘慧霞[1] 李琳[1] 陈宝琅[1] 闫长虹[1] 王旭[1] 孔晓霞[1] 吕昆[1] 

机构地区：[1]菏泽医学专科学校生理学教研室,山东菏泽274030

出　　处：《辽宁医学院学报》2015年第5期8-11,112,共5页Journal of Liaoning Medical University (LNMU) Bimonthly

基　　金：山东省高校科技计划资助项目;编号:J12LE08

摘　　要：目的观察硫化氢对哇巴因诱发触发性心律失常的作用,并进一步探讨其可能的作用机制。方法采用SD大鼠,建立离体心脏Langendorff主动脉逆行灌流系统。应用1μmol/L哇巴因灌流心脏诱发室性心律失常,分别观察50,100,200μmol/L硫氢化钠(H2S的供体)对心律失常的影响,全程记录心电图的变化。按照Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性测定试剂盒检测各组心肌细胞Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性。结果哇巴因可诱发出稳定的室性心律失常,硫氢化钠可浓度依赖性抑制哇巴因诱发的期前收缩,降低室速和室颤的发生率(P<0.05),哇巴因抑制Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活性,硫氢化钠增加其活性。结论硫化氢有抗心律失常的作用,其机制可能与增强Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶的活性有关。Objective To observe hydrogen sulfide＇s effect on ouabain - induced arrhythmias and further explore its possible mechanism. Methods Healthy Sprague - Dawley rats were used to establish a Langen - dorff retrograde perfusion system. Arrhythmia was elicited in isolated rat hearts by the perfusion of 1 Ixmol/L Ouabain, sodium hydrosulfide （50, 100, 200 Ixmol/L） was given im- mediately after the development of first sinus arrhythmia respectively. The arrhythmias were monitored and compared by simultaneous ECG recording, and the Na ＋ - K ＋ - ATP and Ca2 ＋ - Mg2 ＋ - ATP of myocardial cell in each group were examined. Results Ouabain could induce stable ventricular arrhythmias. Sodium hydrosulfide inhibited ouabain - induced premature ventricular beats （PVB） in a concentration -dependent manner, and decreased the incidents of ventricular tachycardia （VT） and ventricular fibrillation （VF） （P 〈 0. 05 ）. Ouabain inhibited the activity of Na ＋ - K＋ - ATP and Ca2 ＋ - Mg2＋ - ATP, while sodium hydrosulfide increased the activi- ty. Conclusion Hydrogen Sulfide can perform anti -arrhythmic function and its mechanism may be related to the increasing activity of Na ＋ - K ＋ - ATP and Ca2 ＋ - Mg2＋ - ATP.

关 键 词：硫化氢 哇巴因 心律失常 

分 类 号：R331.3[医药卫生—人体生理学]