腺苷注射液治疗肺挫伤的作用机制  

作　　者：吴卫春[1] 张丽萍[1] 杜峰[1] 粱治[1] 林伟[1] 王胜利[2] 

机构地区：[1]浙江大学医学院附属第二医院余杭分院心胸外科,311100 [2]温州医科大学附属第二医院,325035

出　　处：《浙江临床医学》2015年第11期1854-1856,共3页Zhejiang Clinical Medical Journal

基　　金：杭州市卫生科技计划资助项目（20128029）

摘　　要：目的探讨腺苷注射液对撞击所致肺挫伤的治疗作用机制。方法选取30只健康日本大耳兔，随机分为对照组和腺苷注射液治疗组，各15只。用BIM-Ⅱ型生物撞击机制备兔肺挫伤模型。分别于撞击前10min、撞击后1h、3h、5h、7h采集颈总动脉血栓测丙二醛（MDA）、白介素．10（IL-10）的浓度；于伤后7h取下右肺，测定肺组织中髓过氧化物酶（MPO）含量；测定支气管肺泡灌洗液中的肿瘤坏死因子-α（TNF-α）含量和肺泡表面活性物质（PS）水平；解剖观察肺组织和病理改变。结果对照组血浆MDA浓度、肺组织中MPO含量、支气管肺泡灌洗液中TNF-α浓度、NE活性较腺苷组显著升高，而腺苷组血浆IL-10浓度和支气管肺泡灌洗液中总磷脂TPL含量较对照组明显高，同时腺苷治疗组肺组织的病理损害明显较对照组减轻。结论腺苷注射液对肺挫伤有治疗作用，其机制可能与调节肺挫伤早期炎性因子浓度有关。Objective To investigate the mechanism of adenosine injection in the treatment of lung contusion following chest impact. Methods Thirty healthy Japan big-ear rabbits were randomly assigned to the lung contusion control group （ control group, n=15 ） and adenosine injection group （ adenosine group, n=15 ） .The rabbits with lung contusion were induced by BIM-Ⅱ Horizontal Bioimpact Machine. Blood samples were taken from common carotid artery of both groups to measure the levels of plasma malondialdehyde （ MDA ） and IL-10 at 10 minutes before impact andl, 3, 5, 7 hours after impact.Rabbits were dissected to observe the gross and micmpathological changes of the lung at 7 hours after impact, the lung tissue myeloperoxidase （ MPO ） acticity and tumor necrosis factor-A （ TNF- α ） and elastase （ NE ） contents in bronchalveor lavage fluid （ BALF ） were obtained. Results The control group showed a distinct increasing of plasma MDA and the lung tissue MPO acticity and TNF- α and NE contents in BALF, The morphological observation revealed severe lung tissue injury as well. The adenosine group showed a distinict inreasing of plasma IL-10 and TPL contents in BALE Conclusion Adenosine injection has therapeutic effect on lung contusion by regulating inflammatory factor levels in the early period after lung contusion.

关 键 词：胸部损伤 肺 腺苷注射液 

分 类 号：R927.2[医药卫生—药学]