HPLC-FLD柱前衍生法同时测定烟草中黄曲霉毒素B_1、B_2、G_1和G_2  

Simultaneous Determination of Aflatoxins B_1, B_2, G_1 and G_2 in Tobacco by HPLC-FLD Pre-column Derivatization Method

作　　者：王康 李韵 肖少红 

机构地区：[1]湖北省烟草产品质量监督检验站,武汉市硚口区宝丰路6号430030

出　　处：《烟草科技》2015年第10期57-61,共5页Tobacco Science & Technology

摘　　要：通过溶剂萃取、免疫亲和柱纯化富集、三氟乙酸柱前衍生、高效液相色谱（HPLC）法分离及荧光检测器检测,建立了同时测定烟草及烟草制品中黄曲霉毒素B1、B2、G1和G2的免疫亲和检测方法。结果表明：1该方法可在20 min内完成测定,4种目标物能够得到很好的分离,线性关系良好,相关系数r值均大于0.99。2方法的回收率为85%-117%,相对标准偏差为0.2%-9.4%（n=6）,其中B1的检出限和定量限分别为0.10和0.34μg/kg。An immunoaffinity detection method for simultaneously determining the aflatoxins B1, B2, G1 and G2 in tobacco and tobacco products was developed via solvent extraction of sample, purifying andconcentrating on immunoaffinity column, pre-column derivatization by trifluoroacetic acid, separation byHPLC, and detection by fluorescence detector. The results showed that： 1） The determination could becompleted within 20 minutes, the four target aflatoxins were well separated and exhibited good linearrelations with correlation coefficients r 0.99. 2） The recoveries of the method ranged from 85% to 117% with the relative standard deviation（RSD） of 0.2%-9.4%（n=6）, and the limits of detection andquantification of aflatoxin B1 were 0.10 and 0.34 μg/kg, respectively.

关 键 词：烟草 黄曲酶毒素 柱前衍生 高效液相色谱法(HPLC) 

分 类 号：TS411.1[农业科学—烟草工业]